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IPO5 antibody [N1N2], N-term

Anti-IPO5 antibody [N1N2], N-term used in Western Blot (WB). GTX114515
Anti-IPO5 antibody [N1N2], N-term used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX114515
Anti-IPO5 antibody [N1N2], N-term used in Western Blot (WB). GTX114515

Cat. No. GTX114515

Reference 1

Host

 Rabbit

Clonality

 Polyclonal

Isotype

 IgG

Application

 WB, ICC/IF

Reactivity

 Human, Mouse
Package
100 μl ($399),
25 μl ($169)
See all IPO5 products

APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Recommended Dilution
WB 1:500-1:3000
ICC/IF 1:100-1:1000
Not tested in other applications.

Calculated MW

124 kDa. ( Note )

Positive Control

Mouse brain , HeLa , NT2D1

Predict Reactivity

Bovine, Pig, Xenopus tropicalis, Chimpanzee(>80% identity)

PROPERTIES

Form

Liquid

Buffer

0.1M Tris, 0.1M Glycine, 20% Glycerol

Preservative

0.01% Thimerosal

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

0.78 mg/ml (Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

Recombinant protein encompassing a sequence within the N-terminus region of human IPO5. The exact sequence is proprietary.

Purification

Purified by antigen-affinity chromatography.

Conjugation

Unconjugated

RRID

AB_10619530

Note

For laboratory research use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

Purchasers shall not, and agree not to enable third parties to, analyze, copy, reverse engineer or otherwise attempt to determine the structure or sequence of the product.

TARGET

Synonyms

importin 5 , IMB3 , KPNB3 , Pse1 , RANBP5 , imp5

Cellular Localization

Cytoplasm , Nucleus , nucleolus

Background

Nucleocytoplasmic transport, a signal- and energy-dependent process, takes place through nuclear pore complexes embedded in the nuclear envelope. The import of proteins containing a nuclear localization signal (NLS) requires the NLS import receptor, a heterodimer of importin alpha and beta subunits also known as karyopherins. Importin alpha binds the NLS-containing cargo in the cytoplasm and importin beta docks the complex at the cytoplasmic side of the nuclear pore complex. In the presence of nucleoside triphosphates and the small GTP binding protein Ran, the complex moves into the nuclear pore complex and the importin subunits dissociate. Importin alpha enters the nucleoplasm with its passenger protein and importin beta remains at the pore. Interactions between importin beta and the FG repeats of nucleoporins are essential in translocation through the pore complex. The protein encoded by this gene is a member of the importin beta family. [provided by RefSeq]

Database

Research Area

DATA IMAGES

Anti-IPO5 antibody [N1N2], N-term used in Western Blot (WB). GTX114515

GTX114515 WB Image

Sample (30 ug of whole cell lysate)
A: NT2D1
B: Hela
5% SDS PAGE
GTX114515 diluted at 1:2000

Anti-IPO5 antibody [N1N2], N-term used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX114515

GTX114515 ICC/IF Image

Immunofluorescence analysis of paraformaldehyde-fixed HeLa, using IPO5(GTX114515) antibody at 1:500 dilution.

Anti-IPO5 antibody [N1N2], N-term used in Western Blot (WB). GTX114515

GTX114515 WB Image

Sample (50 ug of whole cell lysate)
A: Mouse brain
7.5% SDS PAGE
GTX114515 diluted at 1:1000

REFERENCE

Application Reference

Bodnar-Wachtel M et al. Life Sci Alliance 2023; 6 (4) Inflammasome-independent NLRP3 function enforces ATM activity in response to genotoxic stress.
Application : WB
Reactivity : Human
Submit a Reference

REVIEW

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SDS
Glycerol.pdf
Thimerosal.pdf
Package List Price ($)
$ 399
$ 169