GeneTex
  • Country / Location Selection

United States (US)

IQGAP1 antibody [JG38-74]

Anti-IQGAP1 antibody [JG38-74] used in Western Blot (WB). GTX01158
Anti-IQGAP1 antibody [JG38-74] used in IHC (Paraffin sections) (IHC-P). GTX01158
Anti-IQGAP1 antibody [JG38-74] used in Flow cytometry (FACS). GTX01158
Anti-IQGAP1 antibody [JG38-74] used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX01158
Anti-IQGAP1 antibody [JG38-74] used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX01158
Anti-IQGAP1 antibody [JG38-74] used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX01158
Anti-IQGAP1 antibody [JG38-74] used in IHC (Paraffin sections) (IHC-P). GTX01158
Anti-IQGAP1 antibody [JG38-74] used in IHC (Paraffin sections) (IHC-P). GTX01158
Anti-IQGAP1 antibody [JG38-74] used in IHC (Paraffin sections) (IHC-P). GTX01158

Cat No. GTX01158

Host

Rabbit

Clonality

Monoclonal

Clone Name

JG38-74

Isotype

IgG

Application

WB, ICC/IF, IHC-P, FACS

Reactivity

Human, Mouse
Package
100 μl ($369)

APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Dilution
WB 1:500-1:2000
ICC/IF 1:50-1:200
IHC-P 1:50-1:200
FACS 1:50-1:100
Not tested in other applications.

Calculated MW

189 kDa. ( Note )

PROPERTIES

Form

Liquid

Buffer

1x TBS (pH7.4), 1% BSA, 40% glycerol, 0.05% sodium azide

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

1 mg/ml (Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

Recombinant protein

Purification

Protein A purified

Conjugation

Unconjugated

Note

For laboratory use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

TARGET

Synonyms

IQ motif containing GTPase activating protein 1 , HUMORFA01 , IQGAP1 , SAR1 , p195

Cellular Localization

Cell membrane

Background

This gene encodes a member of the IQGAP family. The protein contains four IQ domains, one calponin homology domain, one Ras-GAP domain and one WW domain. It interacts with components of the cytoskeleton, with cell adhesion molecules, and with several signaling molecules to regulate cell morphology and motility. Expression of the protein is upregulated by gene amplification in two gastric cancer cell lines. [provided by RefSeq, Jul 2008]

Database

Research Area

DATA IMAGES

Anti-IQGAP1 antibody [JG38-74] used in Western Blot (WB). GTX01158

GTX01158 WB Image

Various whole cell extracts (30 μg) were separated by 5% SDS-PAGE, and the membrane was blotted with IQGAP1 antibody [JG38-74] (GTX01158) diluted at 1:500. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody. Corresponding RNA expression data for the same cell lines are based on Human Protein Atlas program.

Anti-IQGAP1 antibody [JG38-74] used in IHC (Paraffin sections) (IHC-P). GTX01158

GTX01158 IHC-P Image

IHC-P analysis of human spleen tissue section using GTX01158 IQGAP1 antibody [JG38-74].

Anti-IQGAP1 antibody [JG38-74] used in Flow cytometry (FACS). GTX01158

GTX01158 FACS Image

FACS analysis of SiHa cells using GTX01158 IQGAP1 antibody [JG38-74].
Purple : primary antibody
Yellow : unlabelled control
Dilution : 1:100

Anti-IQGAP1 antibody [JG38-74] used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX01158

GTX01158 ICC/IF Image

ICC/IF analysis of PFA-fixed A431 cells using GTX01158 IQGAP1 antibody [JG38-74].
Green : primary antibody
Blue : DAPI
Permeabilization : 0.25% Triton X-100 in PBS

Anti-IQGAP1 antibody [JG38-74] used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX01158

GTX01158 ICC/IF Image

ICC/IF analysis of PFA-fixed HeLa cells using GTX01158 IQGAP1 antibody [JG38-74].
Green : primary antibody
Blue : DAPI
Permeabilization : 0.25% Triton X-100 in PBS

Anti-IQGAP1 antibody [JG38-74] used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX01158

GTX01158 ICC/IF Image

ICC/IF analysis of PFA-fixed SiHa cells using GTX01158 IQGAP1 antibody [JG38-74].
Green : primary antibody
Blue : DAPI
Permeabilization : 0.25% Triton X-100 in PBS

Anti-IQGAP1 antibody [JG38-74] used in IHC (Paraffin sections) (IHC-P). GTX01158

GTX01158 IHC-P Image

IHC-P analysis of mouse colon tissue section using GTX01158 IQGAP1 antibody [JG38-74].

Anti-IQGAP1 antibody [JG38-74] used in IHC (Paraffin sections) (IHC-P). GTX01158

GTX01158 IHC-P Image

IHC-P analysis of human lung cancer tissue section using GTX01158 IQGAP1 antibody [JG38-74].

Anti-IQGAP1 antibody [JG38-74] used in IHC (Paraffin sections) (IHC-P). GTX01158

GTX01158 IHC-P Image

IHC-P analysis of human placenta tissue section using GTX01158 IQGAP1 antibody [JG38-74].

REFERENCE

There are currently no references for IQGAP1 antibody [JG38-74] (GTX01158). Be the first to share your publications with this product.

REVIEW

There are currently no reviews for IQGAP1 antibody [JG38-74] (GTX01158). Be the first to share your experience with this product.
SDS
Glycerol.pdf
Sodium Azide.pdf