Application Note
*Optimal dilutions/concentrations should be determined by the researcher.
| Application |
Dilution |
| 1 - 2 μg/mL |
| 5 μg/mL |
| Assay dependent |
Not tested in other applications.
Calculated MW
Positive Control
SK-N-SH Cell Lysate(GTX23956)
Specificity/Sensitivity
At least two isoforms of IQSEC2 are known to exist; this antibody will only detect the larger isoform. IQSEC2 antibody is predicted to not cross-react with IQSEC1.
Form
Liquid
Buffer
PBS with 0.02% sodium azide.
Storage
Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.
Concentration
1mg/ml
Antigen Species
Human
Immunogen
IQSEC2 antibody was raised against an 18 amino acid peptide near the carboxy terminus of human IQSEC2.The immunogen is located within amino acids 1380 - 1430 of IQSEC2.
Purification
Purified by antigen-affinity chromatography
Conjugation
Unconjugated
Note
For In vitro laboratory use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.
Synonyms
BRAG1 Antibody , IQ motif and Sec7 domain 2 Antibody , MRX1 Antibody
Cellular Localization
Cytoplasm
Background
This gene encodes a guanine nucleotide exchange factor for the ARF family of small GTP-binding proteins. The encoded protein is a component of the postsynaptic density at excitatory synapses, and may play a critical role in cytoskeletal and synaptic organization through the activation of selected ARF substrates including ARF1 and ARF6. Mutations in this gene have been implicated in nonsyndromic X-linked mental retardation. Alternatively spliced transcript variants encoding multiple isoforms have been observed for this gene. [provided by RefSeq, Aug 2011]
Database
