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IRAK1 antibody

Anti-IRAK1 antibody used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX31253
Anti-IRAK1 antibody used in Western Blot (WB). GTX31253
Anti-IRAK1 antibody used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX31253

Cat No. GTX31253

Host

Rabbit

Clonality

Polyclonal

Isotype

IgG

Application

WB, ICC/IF, IP, ELISA

Reactivity

Human, Mouse, Rat
Package
100 μg ($339)

APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Dilution
WB 1 μg/mL
ICC/IF 10 μg/mL
IP 2 - 4 μg
ELISA Assay dependent
Not tested in other applications.

Calculated MW

77 kDa. ( Note )

Positive Control

HeLa Cell Slide(GTX25529) , THP-1 Cell Lysate(GTX27913) , HeLa Cell Lysate

Specificity/Sensitivity

At least three isoforms of IRAK are known to exist; this antibody will detect all three isoforms. IRAK antibody is predicted to not cross-react with other members of the IRAK protein family.

PROPERTIES

Form

Liquid

Buffer

PBS with 0.02% sodium azide.

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

1 mg/ml (Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

IRAK antibody was raised against a peptide corresponding to 13 amino acids near the carboxy terminus of human IRAK.The immunogen is located within the last 50 amino acids of IRAK.

Purification

Purified by antigen-affinity chromatography

Conjugation

Unconjugated

Note

For laboratory use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

TARGET

Synonyms

interleukin 1 receptor associated kinase 1 , IRAK , pelle

Cellular Localization

Cytoplasm,Nucleus

Background

This gene encodes the interleukin-1 receptor-associated kinase 1, one of two putative serine/threonine kinases that become associated with the interleukin-1 receptor (IL1R) upon stimulation. This gene is partially responsible for IL1-induced upregulation of the transcription factor NF-kappa B. Alternatively spliced transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq, Jul 2008]

Database

Research Area

DATA IMAGES

Anti-IRAK1 antibody used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX31253

GTX31253 ICC/IF Image

ICC/IF analysis of HeLa cells using GTX31253 IRAK1 antibody.
Working concentration : 20 μg/ml

Anti-IRAK1 antibody used in Western Blot (WB). GTX31253

GTX31253 WB Image

WB analysis of THP-1 (THP) and HeLa (HL) whole cell lysates using GTX31253 IRAK1 antibody.
Working concentration : 1 μg/ml

Anti-IRAK1 antibody used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX31253

GTX31253 ICC/IF Image

ICC/IF analysis of HeLa cells using GTX31253 IRAK1 antibody.
Working concentration : 10 μg/ml

REFERENCE

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REVIEW

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SDS
PBS.pdf
Sodium Azide.pdf
Package List Price ($)
$ 339