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IRAK1 antibody

Anti-IRAK1 antibody used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX31253
Anti-IRAK1 antibody used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX31253
Anti-IRAK1 antibody used in Western Blot (WB). GTX31253

Cat. No. GTX31253

Reference 1

Host

 Rabbit

Clonality

 Polyclonal

Isotype

 IgG

Application

 WB, ICC/IF, IP, ELISA

Reactivity

 Human, Mouse, Rat
Package
100 μg ($399)
See all IRAK1 products

APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Recommended Dilution
WB 1 μg/mL
ICC/IF 10 μg/mL
IP 2 - 4 μg
ELISA Assay dependent
Not tested in other applications.

Calculated MW

77 kDa. ( Note )

Positive Control

HeLa Cell Slide(GTX25529) , THP-1 Cell Lysate(GTX27913) , HeLa Cell Lysate

Product Note

At least three isoforms of IRAK are known to exist; this antibody will detect all three isoforms. IRAK antibody is predicted to not cross-react with other members of the IRAK protein family.

PROPERTIES

Form

Liquid

Buffer

PBS

Preservative

0.02% Sodium azide

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

1 mg/ml (Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

IRAK antibody was raised against a peptide corresponding to 13 amino acids near the carboxy terminus of human IRAK.The immunogen is located within the last 50 amino acids of IRAK.

Purification

Purified by antigen-affinity chromatography

Conjugation

Unconjugated

Note

For laboratory research use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

Purchasers shall not, and agree not to enable third parties to, analyze, copy, reverse engineer or otherwise attempt to determine the structure or sequence of the product.

TARGET

Synonyms

interleukin 1 receptor associated kinase 1 , IRAK , pelle

Cellular Localization

Cytoplasm,Nucleus

Background

This gene encodes the interleukin-1 receptor-associated kinase 1, one of two putative serine/threonine kinases that become associated with the interleukin-1 receptor (IL1R) upon stimulation. This gene is partially responsible for IL1-induced upregulation of the transcription factor NF-kappa B. Alternatively spliced transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq, Jul 2008]

Database

Research Area

DATA IMAGES

Anti-IRAK1 antibody used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX31253

GTX31253 ICC/IF Image

ICC/IF analysis of HeLa cells using GTX31253 IRAK1 antibody.
Working concentration : 20 μg/ml

Anti-IRAK1 antibody used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX31253

GTX31253 ICC/IF Image

ICC/IF analysis of HeLa cells using GTX31253 IRAK1 antibody.
Working concentration : 10 μg/ml

Anti-IRAK1 antibody used in Western Blot (WB). GTX31253

GTX31253 WB Image

WB analysis of THP-1 (THP) and HeLa (HL) whole cell lysates using GTX31253 IRAK1 antibody.
Working concentration : 1 μg/ml

REFERENCE

Application Reference

Han P et al. Int J Mol Sci 2023; 24 (8) MicroRNA-146b-5p Suppresses Pro-Inflammatory Mediator Synthesis via Targeting TRAF6, IRAK1, and RELA in Lipopolysaccharide-Stimulated Human Dental Pulp Cells.
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SDS
PBS.pdf
Sodium Azide.pdf
Package List Price ($)
$ 399