GeneTex
United States (US)

IRAK1 antibody

GTX31253_74_ICC-IF_20180221_18121410_147.jpg
GTX31253_2456_WB_20180221_18121410_558.jpg
GTX31253_73_ICC-IF_20180221_18121410_703.jpg

Cat No. GTX31253

Host Rabbit
Clonality Polyclonal
Isotype IgG
Application WB, ICC/IF, IP, ELISA
Reactivity Human, Mouse, Rat
APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Dilution
WB 1 μg/mL
ICC/IF 10 μg/mL
IP 2 - 4 μg
ELISA Assay dependent
Not tested in other applications.

Calculated MW

77 kDa. ( Note )

Positive Control

HeLa Cell Slide(GTX25529) , THP-1 Cell Lysate(GTX27913) , HeLa Cell Lysate

Specificity/Sensitivity

At least three isoforms of IRAK are known to exist; this antibody will detect all three isoforms. IRAK antibody is predicted to not cross-react with other members of the IRAK protein family.
PROPERTIES

Form

Liquid

Buffer

PBS with 0.02% sodium azide.

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

1 mg/ml (Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

IRAK antibody was raised against a peptide corresponding to 13 amino acids near the carboxy terminus of human IRAK.The immunogen is located within the last 50 amino acids of IRAK.

Purification

Purified by antigen-affinity chromatography

Conjugation

Unconjugated

Note

For laboratory use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.
TARGET

Synonyms

Interleukin 1 Receptor Associated Kinase 1,Irak,Pelle,Irak1

Background

This gene encodes the interleukin-1 receptor-associated kinase 1, one of two putative serine/threonine kinases that become associated with the interleukin-1 receptor (IL1R) upon stimulation. This gene is partially responsible for IL1-induced upregulation of the transcription factor NF-kappa B. Alternatively spliced transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq, Jul 2008]

Database

Research Area

DATA IMAGES
GTX31253_74_ICC-IF_20180221_18121410_147.jpg

GTX31253 ICC/IF Image

ICC/IF analysis of HeLa cells using GTX31253 IRAK1 antibody.
Working concentration : 20 μg/ml

GTX31253_2456_WB_20180221_18121410_558.jpg

GTX31253 WB Image

WB analysis of THP-1 (THP) and HeLa (HL) whole cell lysates using GTX31253 IRAK1 antibody.
Working concentration : 1 μg/ml

GTX31253_73_ICC-IF_20180221_18121410_703.jpg

GTX31253 ICC/IF Image

ICC/IF analysis of HeLa cells using GTX31253 IRAK1 antibody.
Working concentration : 10 μg/ml

Package List Price ($)
$ 329