GeneTex
United States (US)

IRAK3 antibody [N1C1]

GTX104227_40093_20180323_WB_B_19011506_616.jpg
GTX104227_IFA_19011506_375.jpg

Cat No. GTX104227

Host Rabbit
Clonality Polyclonal
Isotype IgG
Application WB, ICC/IF
Reactivity Human
APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Dilution
WB 1:500-1:3000
ICC/IF 1:100-1:1000
Not tested in other applications.

Calculated MW

68 kDa. ( Note )

Positive Control

DDDDK-tagged IRAK3-transfected 293T

Predict Reactivity

Mouse, Bovine(>80% identity)
PROPERTIES

Form

Liquid

Buffer

0.1M Tris, 0.1M Glycine, 10% Glycerol (pH7). 0.01% Thimerosal was added as a preservative.

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

0.22mg/ml(Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

Recombinant protein encompassing a sequence within the center region of human IRAK3. The exact sequence is proprietary.

Purification

Purified by antigen-affinity chromatography.

Conjugation

Unconjugated

Note

For laboratory use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.
TARGET

Synonyms

Interleukin 1 Receptor Associated Kinase 3,Asrt5,Irakm,Irak3

Background

This gene encodes a member of the interleukin-1 receptor-associated kinase protein family. Members of this family are essential components of the Toll/IL-R immune signal transduction pathways. This protein is primarily expressed in monocytes and macrophages and functions as a negative regulator of Toll-like receptor signaling. Mutations in this gene are associated with a susceptibility to asthma. Alternate splicing results in multiple transcript variants. [provided by RefSeq]

Database

Research Area

DATA IMAGES
GTX104227_40093_20180323_WB_B_19011506_616.jpg

GTX104227 WB Image

Non-transfected (–) and transfected (+) 293T whole cell extracts (30 µg) were separated by 7.5% SDS-PAGE, and the membrane was blotted with IRAK3 antibody [N1C1] (GTX104227) diluted at 1:500. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

GTX104227_IFA_19011506_375.jpg

GTX104227 ICC/IF Image

Immunofluorescence analysis of paraformaldehyde-fixed HeLa, using IRAK3(GTX104227) antibody at 1:200 dilution.

SDS
Glycerol.pdf
Thimerosal.pdf
Package List Price ($)
$ 319
$ 169