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IRAK3 antibody

Anti-IRAK3 antibody used in Western Blot (WB). GTX31265
Anti-IRAK3 antibody used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX31265
Anti-IRAK3 antibody used in IHC (Paraffin sections) (IHC-P). GTX31265
Anti-IRAK3 antibody used in IHC (Paraffin sections) (IHC-P). GTX31265
Anti-IRAK3 antibody used in IHC (Paraffin sections) (IHC-P). GTX31265

Cat No. GTX31265

Host

Rabbit

Clonality

Polyclonal

Isotype

IgG

Application

WB, ICC/IF, IHC-P, ELISA

Reactivity

Human, Mouse, Rat
Package
100 μg ($339)

APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Dilution
WB 1 μg/mL
ICC/IF Assay dependent
IHC-P 2 μg/mL
ELISA Assay dependent
Not tested in other applications.

Calculated MW

68 kDa. ( Note )

Positive Control

Mouse Spleen Tissue Lysate(GTX27937) , Rat Kidney Tissue Lysate(GTX27944) , Human Spleen Tissue Slide

Specificity/Sensitivity

IRAK-M antibody is predicted to have no cross reactivity to other members of the IRAK family.

PROPERTIES

Form

Liquid

Buffer

PBS with 0.02% sodium azide.

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

1 mg/ml (Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

IRAK3 antibody was raised against a peptide corresponding to 16 amino acids near the carboxy terminus of human IRAK3.The immunogen is located within the last 50 amino acids of IRAK3.

Purification

Purified by antigen-affinity chromatography

Conjugation

Unconjugated

Note

For laboratory use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

TARGET

Synonyms

interleukin 1 receptor associated kinase 3 , ASRT5 , IRAKM

Background

This gene encodes a member of the interleukin-1 receptor-associated kinase protein family. Members of this family are essential components of the Toll/IL-R immune signal transduction pathways. This protein is primarily expressed in monocytes and macrophages and functions as a negative regulator of Toll-like receptor signaling. Mutations in this gene are associated with a susceptibility to asthma. Alternate splicing results in multiple transcript variants. [provided by RefSeq, May 2010]

Database

Research Area

DATA IMAGES

Anti-IRAK3 antibody used in Western Blot (WB). GTX31265

GTX31265 WB Image

WB analysis of (M) mouse spleen and (R) rat liver tissue lysates using GTX31265 IRAK3 antibody.
Working concentration : 1 μg/ml

Anti-IRAK3 antibody used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX31265

GTX31265 ICC/IF Image

ICC/IF analysis of A20 cells using GTX31265 IRAK3 antibody.
Red : Primary antibody
Blue : DAPI
Dilution : 20 μg/ml

Anti-IRAK3 antibody used in IHC (Paraffin sections) (IHC-P). GTX31265

GTX31265 IHC-P Image

IHC-P analysis of human spleen tissue using GTX31265 IRAK3 antibody.
Working concentration : 5 μg/ml

Anti-IRAK3 antibody used in IHC (Paraffin sections) (IHC-P). GTX31265

GTX31265 IHC-P Image

IHC-P analysis of rat liver tissue using GTX31265 IRAK3 antibody.
Working concentration : 2 μg/ml

Anti-IRAK3 antibody used in IHC (Paraffin sections) (IHC-P). GTX31265

GTX31265 IHC-P Image

IHC-P analysis of rat liver tissue using GTX31265 IRAK3 antibody.
Working concentration : 10 μg/ml

REFERENCE

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REVIEW

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SDS
PBS.pdf
Sodium Azide.pdf
Package List Price ($)
$ 339