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IRAK4 antibody [2H9]

Anti-IRAK4 antibody [2H9] used in Western Blot (WB). GTX60479
Anti-IRAK4 antibody [2H9] used in Flow cytometry (FACS). GTX60479
Anti-IRAK4 antibody [2H9] used in IHC (Paraffin sections) (IHC-P). GTX60479
Anti-IRAK4 antibody [2H9] used in IHC (Paraffin sections) (IHC-P). GTX60479
Anti-IRAK4 antibody [2H9] used in ELISA (ELISA). GTX60479
Anti-IRAK4 antibody [2H9] used in Western Blot (WB). GTX60479

Cat. No. GTX60479

Host

 Mouse

Clonality

 Monoclonal

Clone Name

 2H9

Isotype

 IgG1

Application

 WB, IHC-P, FACS, ELISA

Reactivity

 Human, Mouse, Monkey
Package
100 μl ($409)
See all IRAK4 products

APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Recommended Dilution
WB 1/500 - 1/2000
IHC-P 1/200 - 1/1000
FACS 1/200 - 1/400
ELISA 1/10000
Not tested in other applications.

Calculated MW

52 kDa. ( Note )

PROPERTIES

Form

Liquid

Buffer

Ascites

Preservative

0.03% Sodium azide

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Antigen Species

Human

Immunogen

Purified recombinant fragment of human IRAK4 expressed in E. Coli.

Purification

Unpurified

Conjugation

Unconjugated

Note

For laboratory research use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

Purchasers shall not, and agree not to enable third parties to, analyze, copy, reverse engineer or otherwise attempt to determine the structure or sequence of the product.

TARGET

Synonyms

interleukin 1 receptor associated kinase 4 , IPD1 , IRAK-4 , NY-REN-64 , REN64

Cellular Localization

Cytoplasm

Background

This gene encodes a kinase that activates NF-kappaB in both the Toll-like receptor (TLR) and T-cell receptor (TCR) signaling pathways. The protein is essential for most innate immune responses. Mutations in this gene result in IRAK4 deficiency and recurrent invasive pneumococcal disease. Multiple transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq, Aug 2011]

Database

Research Area

DATA IMAGES

Anti-IRAK4 antibody [2H9] used in Western Blot (WB). GTX60479

GTX60479 WB Image

WB analysis of human IRAK4 (AA: 21-198) recombinant protein using GTX60479 IRAK4 antibody [2H9].

Anti-IRAK4 antibody [2H9] used in Flow cytometry (FACS). GTX60479

GTX60479 FACS Image

FACS analysis of HeLa cells using GTX60479 IRAK4 antibody [2H9].
Blue : IRAK4
Red : negative control

Anti-IRAK4 antibody [2H9] used in IHC (Paraffin sections) (IHC-P). GTX60479

GTX60479 IHC-P Image

IHC-P analysis of human kidney cancer tissue using GTX60479 IRAK4 antibody [2H9].

Anti-IRAK4 antibody [2H9] used in IHC (Paraffin sections) (IHC-P). GTX60479

GTX60479 IHC-P Image

IHC-P analysis of human lung cancer tissue using GTX60479 IRAK4 antibody [2H9].

Anti-IRAK4 antibody [2H9] used in ELISA (ELISA). GTX60479

GTX60479 ELISA Image

ELISA analysis of antigen using GTX60479 IRAK4 antibody [2H9].
Red : Control antigen 100ng
Purple : Antigen 10ng
Green : Antigen 50ng
Blue : Antigen 100ng

Anti-IRAK4 antibody [2H9] used in Western Blot (WB). GTX60479

GTX60479 WB Image

WB analysis of THP-1 (1), HeLa (2), K562 (3), MCF-7 (4), RAW264.7 (5), Jurkat (6) and Cos7 (7) cell lysate using GTX60479 IRAK4 antibody [2H9].

REFERENCE

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REVIEW

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SDS
Sodium Azide.pdf
Package List Price ($)
$ 409