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IRAK4 antibody, N-term

Anti-IRAK4 antibody, N-term used in Western Blot (WB). GTX89550
Anti-IRAK4 antibody, N-term used in IHC (Paraffin sections) (IHC-P). GTX89550
Anti-IRAK4 antibody, N-term used in IHC (Paraffin sections) (IHC-P). GTX89550

Cat. No. GTX89550

Host

 Goat

Clonality

 Polyclonal

Isotype

 IgG

Application

 WB, IHC-P

Reactivity

 Human
Package
100 μg ($399)
See all IRAK4 products

APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Recommended Dilution
WB 1-3μg/ml
IHC-P 3.75μg/ml
Not tested in other applications.

Calculated MW

52 kDa. ( Note )

Positive Control

The peptide used to generate this antibody is available for purchase (GTX89550-PEP).

Predict Reactivity

Mouse(>80% identity)

PROPERTIES

Form

Liquid

Buffer

TBS, 0.5% BSA

Preservative

0.02% Sodium azide

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

0.50 mg/ml (Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

Peptide with sequence NKPITPSTYVRC, from the N Terminus of the protein sequence according to NP_057207.2; NP_001107654.1.

Purification

Purified by ammonium sulphate precipitation followed by antigen affinity chromatography

Conjugation

Unconjugated

Note

For laboratory research use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

Purchasers shall not, and agree not to enable third parties to, analyze, copy, reverse engineer or otherwise attempt to determine the structure or sequence of the product.

TARGET

Synonyms

interleukin 1 receptor associated kinase 4 , IPD1 , IRAK-4 , NY-REN-64 , REN64

Cellular Localization

Cytoplasm

Background

This gene encodes a kinase that activates NF-kappaB in both the Toll-like receptor (TLR) and T-cell receptor (TCR) signaling pathways. The protein is essential for most innate immune responses. Mutations in this gene result in IRAK4 deficiency and recurrent invasive pneumococcal disease. Multiple transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq, Aug 2011]

Database

Research Area

DATA IMAGES

Anti-IRAK4 antibody, N-term used in Western Blot (WB). GTX89550

GTX89550 WB Image

WB analysis of HeLa lysate using GTX89550 IRAK4 antibody, N-term.
Dilution : 1μg/ml
Loading : 35μg protein in RIPA buffer

Anti-IRAK4 antibody, N-term used in IHC (Paraffin sections) (IHC-P). GTX89550

GTX89550 IHC-P Image

IHC-P analysis of human liver using GTX89550 IRAK4 antibody, N-term.
Antigen retrieval : citrate buffer pH 6
Dilution : 3.75μg/ml

Anti-IRAK4 antibody, N-term used in IHC (Paraffin sections) (IHC-P). GTX89550

GTX89550 IHC-P Image

IHC-P analysis of human heart using GTX89550 IRAK4 antibody, N-term.
Antigen retrieval : citrate buffer pH 6
Dilution : 3.75μg/ml

REFERENCE

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REVIEW

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SDS
Sodium Azide.pdf
Package List Price ($)
$ 399