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IRAK4 antibody

Anti-IRAK4 antibody used in Western Blot (WB). GTX113323
Anti-IRAK4 antibody used in IHC (Paraffin sections) (IHC-P). GTX113323
Anti-IRAK4 antibody used in IHC (Paraffin sections) (IHC-P). GTX113323

Cat. No. GTX113323

Host

 Rabbit

Clonality

 Polyclonal

Isotype

 IgG

Application

 WB, IHC-P

Reactivity

 Human
Package
100 μl ($399),
25 μl ($169)
See all IRAK4 products

APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Recommended Dilution
WB 1:500-1:3000
IHC-P 1:100-1:1000
Not tested in other applications.

Calculated MW

52 kDa. ( Note )

Positive Control

H1299

Predict Reactivity

Mouse, Rat, Sheep, Bovine, Pig, Rhesus Monkey(>80% identity)

PROPERTIES

Form

Liquid

Buffer

0.1M Tris, 0.1M Glycine, 10% Glycerol

Preservative

0.01% Thimerosal

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

0.54 mg/ml (Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

Recombinant protein encompassing a sequence within the center region of human IRAK4. The exact sequence is proprietary.

Purification

Purified by antigen-affinity chromatography.

Conjugation

Unconjugated

RRID

AB_2037276

Note

For laboratory research use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

Purchasers shall not, and agree not to enable third parties to, analyze, copy, reverse engineer or otherwise attempt to determine the structure or sequence of the product.

TARGET

Synonyms

interleukin 1 receptor associated kinase 4 , IPD1 , IRAK-4 , NY-REN-64 , REN64

Background

This gene encodes a kinase that activates NF-kappaB in both the Toll-like receptor (TLR) and T-cell receptor (TCR) signaling pathways. The protein is essential for most innate immune responses. Mutations in this gene result in IRAK4 deficiency and recurrent invasive pneumococcal disease. Multiple transcript variants encoding different isoforms have been found for this gene. [supplied by RefSeq]

Database

Research Area

DATA IMAGES

Anti-IRAK4 antibody used in Western Blot (WB). GTX113323

GTX113323 WB Image

Sample (30 ug of whole cell lysate)
A: H1299
10% SDS PAGE
GTX113323 diluted at 1:1000

Anti-IRAK4 antibody used in IHC (Paraffin sections) (IHC-P). GTX113323

GTX113323 IHC-P Image

IRAK4 antibody detects IRAK4 protein at cytosol on human gastric cancer by immunohistochemical analysis.
Sample: Paraffin-embedded human gastric cancer.
IRAK4 antibody (GTX113323) dilution: 1:500.

Antigen Retrieval: Trilogy™ (EDTA based, pH 8.0) buffer, 15min

Anti-IRAK4 antibody used in IHC (Paraffin sections) (IHC-P). GTX113323

GTX113323 IHC-P Image

IRAK4 antibody detects IRAK4 protein at cytosol on human colon carcinoma by immunohistochemical analysis.
Sample: IRAK4 antibody (GTX113323) dilution: 1:250.

Antigen Retrieval: Trilogy™ (EDTA based, pH 8.0) buffer, 15min

REFERENCE

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REVIEW

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SDS
Glycerol.pdf
Thimerosal.pdf
Package List Price ($)
$ 399
$ 169