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IRAK4 antibody

Anti-IRAK4 antibody used in IHC (Paraffin sections) (IHC-P). GTX113324
Anti-IRAK4 antibody used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX113324
Anti-IRAK4 antibody used in Western Blot (WB). GTX113324

Cat No. GTX113324

Host

Rabbit

Clonality

Polyclonal

Isotype

IgG

Application

WB, ICC/IF, IHC-P

Reactivity

Human
Package
100 μl ($329),
25 μl ($169)

APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Dilution
WB 1:500-1:3000
ICC/IF 1:100-1:1000
IHC-P 1:100-1:1000
Not tested in other applications.

Calculated MW

52 kDa. ( Note )

Positive Control

U87-MG

Predict Reactivity

Sheep, Bovine, Pig, Rhesus Monkey(>80% identity)

PROPERTIES

Form

Liquid

Buffer

0.1M Tris, 0.1M Glycine, 20% Glycerol (pH7). 0.01% Thimerosal was added as a preservative.

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

0.76 mg/ml (Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

Recombinant protein encompassing a sequence within the center region of human IRAK4. The exact sequence is proprietary.

Purification

Purified by antigen-affinity chromatography.

Conjugation

Unconjugated

Note

For laboratory use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

TARGET

Synonyms

interleukin 1 receptor associated kinase 4 , IPD1 , IRAK-4 , NY-REN-64 , REN64

Background

This gene encodes a kinase that activates NF-kappaB in both the Toll-like receptor (TLR) and T-cell receptor (TCR) signaling pathways. The protein is essential for most innate immune responses. Mutations in this gene result in IRAK4 deficiency and recurrent invasive pneumococcal disease. Multiple transcript variants encoding different isoforms have been found for this gene. [supplied by RefSeq]

Database

Research Area

DATA IMAGES

Anti-IRAK4 antibody used in IHC (Paraffin sections) (IHC-P). GTX113324

GTX113324 IHC-P Image

Immunohistochemical analysis of paraffin-embedded Hela xenograft, using IRAK4(GTX113324) antibody at 1:500 dilution.
Antigen Retrieval: Trilogy™ (EDTA based, pH 8.0) buffer, 15min

Anti-IRAK4 antibody used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX113324

GTX113324 ICC/IF Image

IRAK4 antibody detects IRAK4 protein at cytoplasm and nucleus by immunofluorescent analysis.
Sample: HeLa cells were fixed in 4% paraformaldehyde for 10 min.
Green: IRAK4 protein stained by IRAK4 antibody (GTX113324) diluted at 1:100.
Blue: Hoechst 33342 staining.
Scale bar = 10 μm.

Anti-IRAK4 antibody used in Western Blot (WB). GTX113324

GTX113324 WB Image

Sample (30 ug of whole cell lysate)
A: U87-MG
10% SDS PAGE
GTX113324 diluted at 1:1000

REFERENCE

There are currently no references for IRAK4 antibody (GTX113324). Be the first to share your publications with this product.

REVIEW

There are currently no reviews for IRAK4 antibody (GTX113324). Be the first to share your experience with this product.
SDS
Glycerol.pdf
Thimerosal.pdf
Package List Price ($)
$ 329
$ 169