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IRAK4 antibody

Anti-IRAK4 antibody used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX25985
Anti-IRAK4 antibody used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX25985
Anti-IRAK4 antibody used in Western Blot (WB). GTX25985

Cat. No. GTX25985

Host

 Rabbit

Clonality

 Polyclonal

Isotype

 IgG

Application

 WB, ICC/IF, ELISA

Reactivity

 Human
Package
100 μg ($479)
See all IRAK4 products

APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Recommended Dilution
WB 1-4μg/ml
ICC/IF 10μg/ml
ELISA Assay dependent
Not tested in other applications.

Calculated MW

52 kDa. ( Note )

Product Note

This antibody specifically recognizes human IRAK-4 (IL-1 Receptor Associated Kinase-4). This antibody does not cross-react with other IRAKs.

PROPERTIES

Form

Liquid

Buffer

PBS

Preservative

0.02% Sodium azide

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

1 mg/ml (Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

A synthetic peptide corresponding to amino acids at the carboxy terminus of human IRAK-4.

Purification

Purified by affinity chromatography

Conjugation

Unconjugated

Note

For laboratory research use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

Purchasers shall not, and agree not to enable third parties to, analyze, copy, reverse engineer or otherwise attempt to determine the structure or sequence of the product.

TARGET

Synonyms

IPD1 , IRAK4 , NYREN64 , REN64 , interleukin 1 receptor associated kinase 4

Cellular Localization

Cytoplasm

Background

This gene encodes a kinase that activates NF-kappaB in both the Toll-like receptor (TLR) and T-cell receptor (TCR) signaling pathways. The protein is essential for most innate immune responses. Mutations in this gene result in IRAK4 deficiency and recurrent invasive pneumococcal disease. Multiple transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq, Aug 2011]

Database

Research Area

DATA IMAGES

Anti-IRAK4 antibody used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX25985

GTX25985 ICC/IF Image

ICC/IF analysis of K562 cells using GTX25985 IRAK4 antibody.
Dilution : 10 μg/ml

Anti-IRAK4 antibody used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX25985

GTX25985 ICC/IF Image

ICC/IF analysis of K562 cells using GTX25985 IRAK4 antibody.
Dilution : 10 μg/ml

Anti-IRAK4 antibody used in Western Blot (WB). GTX25985

GTX25985 WB Image

WB analysis of HeLa cell lysate using GTX25985 IRAK4 antibody.
Dilution : 1μg/ml (Lane A), 2 μg/ml (Lane B), and 4μg/ml (Lane C)

REFERENCE

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REVIEW

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SDS
PBS.pdf
Package List Price ($)
$ 479