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IRE1 alpha (phospho Ser724) antibody

Anti-IRE1 alpha (phospho Ser724) antibody used in Western Blot (WB). GTX130378

Cat. No. GTX130378

Host

Rabbit

Clonality

Polyclonal

Isotype

IgG

Application

WB

Reactivity

Human
Package
100 μl ($399),
25 μl ($169)

APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Recommended Dilution
WB 1:500-1:3000
Not tested in other applications.

Calculated MW

110 kDa. ( Note )

Positive Control

HeLa (100 nM Calyculin A , 30 min)

Predict Reactivity

Mouse, Rat, Chicken, Japanese Medaka(>80% identity)

PROPERTIES

Form

Liquid

Buffer

1XPBS (pH7), 1% BSA, 20% Glycerol

Preservative

0.025% ProClin 300

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

0.18 mg/ml (Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

Carrier-protein conjugated synthetic peptide surrounding phospho Ser724 of human IRE1 alpha. The exact sequence is proprietary.

Purification

Purified by antigen-affinity chromatography.

Conjugation

Unconjugated

RRID

AB_2886250

Note

For laboratory use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

TARGET

Synonyms

endoplasmic reticulum to nucleus signaling 1 , IRE1 , IRE1P , IRE1a , hIRE1p

Cellular Localization

Endoplasmic reticulum membrane; Single-pass type I membrane protein

Background

The protein encoded by this gene is the ER to nucleus signalling 1 protein, a human homologue of the yeast Ire1 gene product. This protein possesses intrinsic kinase activity and an endoribonuclease activity and it is important in altering gene expression as a response to endoplasmic reticulum-based stress signals. [provided by RefSeq]

Database

Research Area

DATA IMAGES

Anti-IRE1 alpha (phospho Ser724) antibody used in Western Blot (WB). GTX130378

GTX130378 WB Image

Untreated (–) and treated (+) HeLa whole cell extracts (5 μg) were separated by 7.5% SDS-PAGE, and the membrane was blotted with IRE1 (phospho Ser724) antibody (GTX130378) diluted at 1:1000.

REFERENCE

There are currently no references for IRE1 alpha (phospho Ser724) antibody (GTX130378). Be the first to share your publications with this product.

REVIEW

There are currently no reviews for IRE1 alpha (phospho Ser724) antibody (GTX130378). Be the first to share your experience with this product.
SDS
PBS.pdf
Glycerol.pdf
Proclin.pdf
Package List Price ($)
$ 399
$ 169