APPLICATION
Application Note
*Optimal dilutions/concentrations should be determined by the researcher.
Application |
Recommended Dilution |
1:500-1:3000 |
Not tested in other applications.
Calculated MW
Positive Control
HeLa (100 nM Calyculin A , 30 min)
Predict Reactivity
Mouse, Rat, Chicken, Japanese Medaka(>80% identity)
PROPERTIES
Form
Liquid
Buffer
1XPBS (pH7), 1% BSA, 20% Glycerol
Preservative
0.025% ProClin 300
Storage
Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.
Concentration
0.18 mg/ml (Please refer to the vial label for the specific concentration.)
Antigen Species
Human
Immunogen
Carrier-protein conjugated synthetic peptide surrounding phospho Ser724 of human IRE1 alpha. The exact sequence is proprietary.
Purification
Purified by antigen-affinity chromatography.
Conjugation
Unconjugated
RRID
AB_2886250
Note
For laboratory use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.
TARGET
Synonyms
endoplasmic reticulum to nucleus signaling 1 , IRE1 , IRE1P , IRE1a , hIRE1p
Cellular Localization
Endoplasmic reticulum membrane; Single-pass type I membrane protein
Background
The protein encoded by this gene is the ER to nucleus signalling 1 protein, a human homologue of the yeast Ire1 gene product. This protein possesses intrinsic kinase activity and an endoribonuclease activity and it is important in altering gene expression as a response to endoplasmic reticulum-based stress signals. [provided by RefSeq]
Database
Research Area
DATA IMAGES
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GTX130378 WB Image
Untreated (–) and treated (+) HeLa whole cell extracts (5 μg) were separated by 7.5% SDS-PAGE, and the membrane was blotted with IRE1 (phospho Ser724) antibody (GTX130378) diluted at 1:1000.
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REFERENCE
There are currently no references for IRE1 alpha (phospho Ser724) antibody (GTX130378). Be the first to share your publications with this product.
REVIEW
There are currently no reviews for IRE1 alpha (phospho Ser724) antibody (GTX130378). Be the first to share your experience with this product.