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IRE1 alpha (phospho Ser724) antibody

Orthogonal
Anti-IRE1 alpha (phospho Ser724) antibody used in Western Blot (WB). GTX132808
Anti-IRE1 alpha (phospho Ser724) antibody used in Western Blot (WB). GTX132808
Anti-IRE1 alpha (phospho Ser724) antibody used in Western Blot (WB). GTX132808
Anti-IRE1 alpha (phospho Ser724) antibody used in Western Blot (WB). GTX132808
Anti-IRE1 alpha (phospho Ser724) antibody used in Western Blot (WB). GTX132808

Cat. No. GTX132808

Reference 7
1

Host

 Rabbit

Clonality

 Polyclonal

Isotype

 IgG

Application

 WB

Reactivity

 Human, Mouse, Rat
Package
100 μl ($419),
25 μl ($169)
See all IRE1 alpha products

APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Recommended Dilution
WB 1:500-1:3000
Not tested in other applications.

Calculated MW

110 kDa. ( Note )

Positive Control

Neuro2A , C8D30 , NIH-3T3 , C2C12 , Rat2

Predict Reactivity

Chicken, Japanese Medaka(>80% identity)

PROPERTIES

Form

Liquid

Buffer

PBS, 1% BSA, 20% Glycerol

Preservative

0.025% ProClin 300

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

0.5 mg/ml (Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

Carrier-protein conjugated synthetic peptide surrounding phospho Ser724 of human IRE1 alpha. The exact sequence is proprietary.

Purification

Purified by antigen-affinity chromatography.

Conjugation

Unconjugated

RRID

AB_2886751

Note

For laboratory research use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

Purchasers shall not, and agree not to enable third parties to, analyze, copy, reverse engineer or otherwise attempt to determine the structure or sequence of the product.

TARGET

Synonyms

endoplasmic reticulum to nucleus signaling 1 , IRE1 , IRE1P , IRE1a , hIRE1p

Cellular Localization

Endoplasmic reticulum membrane; Single-pass type I membrane protein

Background

The protein encoded by this gene is the ER to nucleus signalling 1 protein, a human homologue of the yeast Ire1 gene product. This protein possesses intrinsic kinase activity and an endoribonuclease activity and it is important in altering gene expression as a response to endoplasmic reticulum-based stress signals. [provided by RefSeq]

Database

Research Area

DATA IMAGES

Anti-IRE1 alpha (phospho Ser724) antibody used in Western Blot (WB). GTX132808

GTX132808 WB Image

Orthogonal

Untreated (–) and treated (+) Neuro2A whole cell extract (30 μg) were separated by 5% SDS-PAGE, and the membrane was blotted with IRE1 alpha (phospho Ser724) antibody (GTX132808) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody, and the signal was developed with Trident ECL plus-Enhanced.

Anti-IRE1 alpha (phospho Ser724) antibody used in Western Blot (WB). GTX132808

GTX132808 WB Image

Various whole cell extracts (30 μg) were separated by 7.5% SDS-PAGE, and the membrane was blotted with IRE1 alpha (phospho Ser724) antibody (GTX132808) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

Anti-IRE1 alpha (phospho Ser724) antibody used in Western Blot (WB). GTX132808

GTX132808 WB Image

Orthogonal

Untreated (–) and treated (+) HepG2 whole cell extracts (30 μg) were separated by 5% SDS-PAGE, and the membrane was blotted with IRE1 alpha (phospho Ser724) antibody (GTX132808) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

Anti-IRE1 alpha (phospho Ser724) antibody used in Western Blot (WB). GTX132808

GTX132808 WB Image

Various whole cell extracts (30 μg) were separated by 7.5% SDS-PAGE, and the membrane was blotted with IRE1 alpha (phospho Ser724) antibody (GTX132808) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

Anti-IRE1 alpha (phospho Ser724) antibody used in Western Blot (WB). GTX132808

GTX132808 WB Image

The data was published in the journal Oxid Med Cell Longev in 2015. PMID: 25685256

REFERENCE

Application Reference

Kang L et al. Eye Vis (Lond) 2023; 10 (1) : 44 Establishment of a novel ER-stress induced myopia model in mice.
Chen J et al. Front Med (Lausanne) 2023; 10 : 1255121 Bisphenol A exposure triggers endoplasmic reticulum stress pathway leading to ocular axial elongation in mice.
Ikeda SI et al. Nat Commun 2022; 13 (1) : 5859 Scleral PERK and ATF6 as targets of myopic axial elongation of mouse eyes.
Tao J et al. Front Oncol 2022; 12 : 836087 PDIA2 Bridges Endoplasmic Reticulum Stress and Metabolic Reprogramming During Malignant Transformation of Chronic Colitis.
Yu W et al. Acta Pharm Sin B 2022; 12 (5) : 2315-2329 The substitution of SERCA2 redox cysteine 674 promotes pulmonary vascular remodeling by activating IRE1α/XBP1s pathway.
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REVIEW

IRE1 alpha (phospho Ser724) antibody Cat. No. GTX132808

Rating
( Average 5 based on 1 users reviews)
Application
Western Blot(WB)
( Average 5 based on 1 users reviews)
Anti-IRE1 alpha (phospho Ser724) antibody used in Western Blot (WB). GTX132808
Date : Anonymous submitted on 05-Aug-2021
Score :
Application Tested : WB
Sample Species : Hu
Sample : U2OS cells
Amount used : 30 μg
Blocking : 5% non-fat dry milk in TBST, 25°C, 1Hr
Primary Antibody : 1:1000, 4°C, 12Hr
Figure Note : 0-30 μM DOX treatment for 2 h.
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SDS
PBS.pdf
Glycerol.pdf
Proclin.pdf
Package List Price ($)
$ 419
$ 169
Currently in production