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IRE1 alpha (phospho Ser724) antibody

Orthogonal
Anti-IRE1 alpha (phospho Ser724) antibody used in Western Blot (WB). GTX132808
Anti-IRE1 alpha (phospho Ser724) antibody used in Western Blot (WB). GTX132808
Anti-IRE1 alpha (phospho Ser724) antibody used in Western Blot (WB). GTX132808
Anti-IRE1 alpha (phospho Ser724) antibody used in Western Blot (WB). GTX132808

Cat No. GTX132808

Reference 1

Host

 Rabbit

Clonality

 Polyclonal

Isotype

 IgG

Application

 WB

Reactivity

 Human, Mouse, Rat
Package
100 μl ($329),
25 μl ($169)
See all IRE1 alpha products

APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Dilution
WB 1:500-1:3000
Not tested in other applications.

Calculated MW

110 kDa. ( Note )

Positive Control

Neuro2A , C8D30 , NIH-3T3 , C2C12 , Rat2 , HepG2 , HepG2 (3μM Thapsigargin treatment for 12 hr)

Predict Reactivity

Japanese Medaka(>80% identity)

PROPERTIES

Form

Liquid

Buffer

1XPBS, 1% BSA, 20% Glycerol (pH7). 0.025% ProClin 300 was added as a preservative.

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

0.57 mg/ml (Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

Carrier-protein conjugated synthetic peptide corresponding to residues around human IRE1 (phospho Ser724). The exact sequence is proprietary.

Purification

Purified by antigen-affinity chromatography.

Conjugation

Unconjugated

Note

For laboratory use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

TARGET

Synonyms

endoplasmic reticulum to nucleus signaling 1 , IRE1 , IRE1P , IRE1a , hIRE1p

Cellular Localization

Endoplasmic reticulum membrane; Single-pass type I membrane protein

Background

The protein encoded by this gene is the ER to nucleus signalling 1 protein, a human homologue of the yeast Ire1 gene product. This protein possesses intrinsic kinase activity and an endoribonuclease activity and it is important in altering gene expression as a response to endoplasmic reticulum-based stress signals. [provided by RefSeq]

Database

Research Area

DATA IMAGES

Anti-IRE1 alpha (phospho Ser724) antibody used in Western Blot (WB). GTX132808

GTX132808 WB Image

Various whole cell extracts (30 μg) were separated by 7.5% SDS-PAGE, and the membrane was blotted with IRE1 alpha (phospho Ser724) antibody (GTX132808) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

Anti-IRE1 alpha (phospho Ser724) antibody used in Western Blot (WB). GTX132808

GTX132808 WB Image

Various whole cell extracts (30 μg) were separated by 7.5% SDS-PAGE, and the membrane was blotted with IRE1 alpha (phospho Ser724) antibody (GTX132808) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

Anti-IRE1 alpha (phospho Ser724) antibody used in Western Blot (WB). GTX132808

GTX132808 WB Image

Orthogonal

Untreated (–) and treated (+) HepG2 whole cell extracts (30 μg) were separated by 5% SDS-PAGE, and the membrane was blotted with IRE1 alpha (phospho Ser724) antibody (GTX132808) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

Anti-IRE1 alpha (phospho Ser724) antibody used in Western Blot (WB). GTX132808

GTX132808 WB Image

The WB analysis of IRE1 alpha (phospho Ser724) antibody was published by Lien JC and colleagues in the journal Oxid Med Cell Longev in 2015.PMID: 25685256

REFERENCE

Application Reference

Lien JC et al. Oxid Med Cell Longev 2015; 2015 : 453679 Naphthoquinone derivative PPE8 induces endoplasmic reticulum stress in p53 null H1299 cells.
Application : WB
Reactivity : Human
Submit a Reference

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SDS
PBS.pdf
Glycerol.pdf
Proclin.pdf
Package List Price ($)
$ 329
$ 169