IRE1 alpha (phospho Ser724) antibody

Name: IRE1 alpha (phospho Ser724) antibody
Brand: GeneTex
SKU: GTX132808
Price: 399.00 USD
Rating: 5 (2 reviews)

Anti-IRE1 alpha (phospho Ser724) antibody used in Western Blot (WB). GTX132808

Cat. No. GTX132808

Reference 2

Host	Rabbit
Clonality	Polyclonal
Isotype	IgG
Application	WB
Reactivity	Human, Mouse, Rat
Package	100 μl ($399), 25 μl ($169)

See all IRE1 alpha products

APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.

Application	Recommended Dilution
WB	1:500-1:3000

Not tested in other applications.

Calculated MW

110 kDa. ( Note )

Positive Control

Neuro2A , C8D30 , NIH-3T3 , C2C12 , Rat2

Predict Reactivity

Chicken, Japanese Medaka(>80% identity)

PROPERTIES

Form

Liquid

Buffer

1XPBS pH7, 1% BSA, 20% Glycerol

Preservative

0.025% ProClin 300

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

0.5 mg/ml (Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

Carrier-protein conjugated synthetic peptide surrounding phospho Ser724 of human IRE1 alpha. The exact sequence is proprietary.

Purification

Purified by antigen-affinity chromatography.

Conjugation

Unconjugated

RRID

AB_2886751

Note

For laboratory use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

TARGET

Synonyms

endoplasmic reticulum to nucleus signaling 1 , IRE1 , IRE1P , IRE1a , hIRE1p

Cellular Localization

Endoplasmic reticulum membrane; Single-pass type I membrane protein

Background

The protein encoded by this gene is the ER to nucleus signalling 1 protein, a human homologue of the yeast Ire1 gene product. This protein possesses intrinsic kinase activity and an endoribonuclease activity and it is important in altering gene expression as a response to endoplasmic reticulum-based stress signals. [provided by RefSeq]

Database

Gene ID: 2081 ERN1
UniProt: O75460 ERN1

Research Area

DATA IMAGES

GTX132808 WB Image

Untreated (–) and treated (+) HepG2 whole cell extracts (30 μg) were separated by 5% SDS-PAGE, and the membrane was blotted with IRE1 alpha (phospho Ser724) antibody (GTX132808) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

GTX132808 WB Image

Untreated (–) and treated (+) Neuro2A whole cell extract (30 μg) were separated by 5% SDS-PAGE, and the membrane was blotted with IRE1 alpha (phospho Ser724) antibody (GTX132808) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody, and the signal was developed with Trident ECL plus-Enhanced.

GTX132808 WB Image

Various whole cell extracts (30 μg) were separated by 7.5% SDS-PAGE, and the membrane was blotted with IRE1 alpha (phospho Ser724) antibody (GTX132808) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

GTX132808 WB Image

REFERENCE

Application Reference

Choi YK et al. Nutrients 2020; 12 (5) L-Ascorbic Acid Inhibits Breast Cancer Growth by Inducing IRE/JNK/CHOP-Related Endoplasmic Reticulum Stress-Mediated p62/SQSTM1 Accumulation in the Nucleus.

Application : WB

Reactivity : Human

Lien JC et al. Oxid Med Cell Longev 2015; 2015 : 453679 Naphthoquinone derivative PPE8 induces endoplasmic reticulum stress in p53 null H1299 cells.

Application : WB

Reactivity : Human

Submit a Reference

REVIEW