GeneTex
United States (US)

IREB2 antibody, Internal

GTX88335_982_IHC-P_18121411_196.jpg
GTX88335_2375_WB_18121411_912.jpg

Cat No. GTX88335

Host Goat
Clonality Polyclonal
Isotype IgG
Application WB, IHC-P
Reactivity Human
APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Dilution
WB 1-3μg/ml
IHC-P 3-6μg/ml

Note :

Human Kidney shows preferential staining in the cytoplasm of DCT.
Not tested in other applications.

Calculated MW

105 kDa. ( Note )

Positive Control

The peptide used to generate this antibody is available for purchase (GTX88335-PEP).

Predict Reactivity

Mouse, Rat(>80% identity)
PROPERTIES

Form

Liquid

Buffer

Tris saline pH7.3, 0.02% sodium azide, 0.5% BSA.

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

0.50mg/ml

Antigen Species

Human

Immunogen

Peptide with sequence C-SIHYEGSEYKLSHGS, from the internal region of the protein sequence according to NP_004127.1.

Purification

Purified by ammonium sulphate precipitation followed by antigen affinity chromatography

Conjugation

Unconjugated

Note

For In vitro laboratory use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.
TARGET

Synonyms

IRP2 Antibody , ACO3 Antibody , IRP2AD Antibody

Database

Research Area

DATA IMAGES
GTX88335_982_IHC-P_18121411_196.jpg

GTX88335 IHC-P Image

IHC-P analysis of human kidney using GTX88335 IREB2 antibody, Internal.
Antigen retrieval : citrate buffer pH 6
Dilution : 3.8µg/ml

GTX88335_2375_WB_18121411_912.jpg

GTX88335 WB Image

WB analysis of human liver lysate with (B) and without (A) blocking with the immunising peptide using GTX88335 IREB2 antibody, Internal.
Dilution : 0.3µg/ml
Loading : 35µg protein in RIPA buffer