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IRF1 antibody [SR44-08]

Recombinant antibody
Anti-IRF1 antibody [SR44-08] used in Western Blot (WB). GTX01047
Anti-IRF1 antibody [SR44-08] used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX01047
Anti-IRF1 antibody [SR44-08] used in IHC (Paraffin sections) (IHC-P). GTX01047
Anti-IRF1 antibody [SR44-08] used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX01047
Anti-IRF1 antibody [SR44-08] used in Flow cytometry (FACS). GTX01047
Anti-IRF1 antibody [SR44-08] used in IHC (Paraffin sections) (IHC-P). GTX01047

Cat No. GTX01047

Host

Rabbit

Clonality

Monoclonal

Clone Name

SR44-08

Isotype

IgG

Application

WB, ICC/IF, IHC-P, FACS

Reactivity

Human, Mouse
Package
100 μl ($369)

APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Dilution
WB 1:1000-1:2000
ICC/IF 1:50-1:200
IHC-P 1:50-1:200
FACS 1:50-1:100
Not tested in other applications.

Calculated MW

36 kDa. ( Note )

Observed MW

50 kDa. The observed M.W. is based on the following publication. PMID: 21389130

PROPERTIES

Form

Liquid

Buffer

1x TBS (pH7.4), 1% BSA, 40% glycerol, 0.05% sodium azide

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

1 mg/ml (Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

Recombinant protein

Purification

Protein A purified

Conjugation

Unconjugated

Note

For laboratory use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

TARGET

Synonyms

interferon regulatory factor 1,IRF1,MAR

Cellular Localization

Nucleus,Cytoplasm

Background

The protein encoded by this gene is a transcriptional regulator and tumor suppressor, serving as an activator of genes involved in both innate and acquired immune responses. The encoded protein activates the transcription of genes involved in the body's response to viruses and bacteria, playing a role in cell proliferation, apoptosis, the immune response, and DNA damage response. This protein represses the transcription of several other genes. As a tumor suppressor, it both suppresses tumor cell growth and stimulates an immune response against tumor cells. Defects in this gene have been associated with gastric cancer, myelogenous leukemia, and lung cancer. [provided by RefSeq, Aug 2017]

Database

Research Area

DATA IMAGES

Anti-IRF1 antibody [SR44-08] used in Western Blot (WB). GTX01047

GTX01047 WB Image

Various whole cell extracts (30 μg) were separated by 10% SDS-PAGE, and the membrane was blotted with IRF1 antibody [SR44-08] (GTX01047) diluted at 1:500. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.
The observed M.W. is based on the publication: PMID: 21389130

Anti-IRF1 antibody [SR44-08] used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX01047

GTX01047 ICC/IF Image

ICC/IF analysis of PFA-fixed NIH-3T3 cells using GTX01047 IRF1 antibody [SR44-08].
Green : Primary antibody
Blue : DAPI
Permeabilization : 0.25% Triton X-100 in PBS

Anti-IRF1 antibody [SR44-08] used in IHC (Paraffin sections) (IHC-P). GTX01047

GTX01047 IHC-P Image

IHC-P analysis of mouse brain tissue section using GTX01047 IRF1 antibody [SR44-08].

Anti-IRF1 antibody [SR44-08] used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX01047

GTX01047 ICC/IF Image

ICC/IF analysis of PFA-fixed HeLa cells using GTX01047 IRF1 antibody [SR44-08].
Green : Primary antibody
Blue : DAPI
Permeabilization : 0.25% Triton X-100 in PBS

Anti-IRF1 antibody [SR44-08] used in Flow cytometry (FACS). GTX01047

GTX01047 FACS Image

FACS analysis of Jurkat cells using GTX01047 IRF1 antibody [SR44-08].
Blue : Primary antibody
Red : unlabelled control
Dilution : 1:50

Anti-IRF1 antibody [SR44-08] used in IHC (Paraffin sections) (IHC-P). GTX01047

GTX01047 IHC-P Image

IHC-P analysis of human colon cancer tissue section using GTX01047 IRF1 antibody [SR44-08].

REFERENCE

There are currently no references for IRF1 antibody [SR44-08] (GTX01047). Be the first to share your publications with this product.

REVIEW

There are currently no reviews for IRF1 antibody [SR44-08] (GTX01047). Be the first to share your experience with this product.
Package List Price ($)
$ 369