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IRF3 antibody [3F10]

Anti-IRF3 antibody [3F10] used in Western Blot (WB). GTX50058
Anti-IRF3 antibody [3F10] used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX50058

Cat. No. GTX50058

Host

 Mouse

Clonality

 Monoclonal

Clone Name

 3F10

Isotype

 IgG1

Application

 WB, ICC/IF, IP, ELISA

Reactivity

 Human
Package
100 μl ($399)
See all IRF3 products

APPLICATION

Application Note

We recommend the following starting dilutions:
Western Blot: Use at 1:1,000 ~ 2,000.

Optimal working concentrations should be determined experimentally by the end user.

Calculated MW

47 kDa. ( Note )

PROPERTIES

Form

Liquid

Buffer

PBS

Preservative

0.1% Sodium azide

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

1 mg/ml (Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

Recombinant human IRF-3(108-166aa) purified from E. coli

Purification

By protein-G affinity chromatography

Conjugation

Unconjugated

Note

For laboratory research use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

Purchasers shall not, and agree not to enable third parties to, analyze, copy, reverse engineer or otherwise attempt to determine the structure or sequence of the product.

TARGET

Synonyms

interferon regulatory factor 3 , IIAE7

Cellular Localization

Cytoplasm , Nucleus

Background

This gene encodes a member of the interferon regulatory transcription factor (IRF) family. The encoded protein is found in an inactive cytoplasmic form that upon serine/threonine phosphorylation forms a complex with CREBBP. This complex translocates to the nucleus and activates the transcription of interferons alpha and beta, as well as other interferon-induced genes. Alternatively spliced transcript variants encoding multiple isoforms have been observed for this gene. [provided by RefSeq, Nov 2011]

Database

Research Area

DATA IMAGES

Anti-IRF3 antibody [3F10] used in Western Blot (WB). GTX50058

GTX50058 WB Image

Cell lysates of HeLa(40ug) were resolved by SDS-PAGE, transferred to NC membrane and probed with anti-human IRF-3 (1:1,000). Proteins were visualized using a goat anti-mouse secondary antibody conjugated to HRP and an ECL detection system.

Anti-IRF3 antibody [3F10] used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX50058

GTX50058 ICC/IF Image

Immunofluorescence of human HeLa cells stained with IRF-3 (1:500) with Texas-Red and Hoechst 33342 (Blue) for nucleus staining.

REFERENCE

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REVIEW

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SDS
Sodium Azide.pdf
Package List Price ($)
$ 399