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IRF3 antibody [3F10]

Anti-IRF3 antibody [3F10] used in Western Blot (WB). GTX50058
Anti-IRF3 antibody [3F10] used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX50058

Cat No. GTX50058

Host

Mouse

Clonality

Monoclonal

Clone Name

3F10

Isotype

IgG1

Application

WB, ICC/IF, IP, ELISA

Reactivity

Human
Package
100 μl ($319)

APPLICATION

Application Note

We recommend the following starting dilutions:
Western Blot: Use at 1:1,000 ~ 2,000.

Optimal working concentrations should be determined experimentally by the end user.

Calculated MW

47 kDa. ( Note )

PROPERTIES

Form

Liquid

Buffer

Supplied in Phosphate-Buffered Saline (pH 7.4) with 0.1% Sodium Azide.

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

1 mg/ml (Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

Recombinant human IRF-3(108-166aa) purified from E. coli

Purification

By protein-G affinity chromatography

Conjugation

Unconjugated

Note

For laboratory use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

TARGET

Synonyms

Interferon Regulatory Factor 3 , Iiae7 , Irf3

Cellular Localization

Shuttles between cytoplasmic and nuclear compartments, with export being the prevailing effect. When activated, IRF3 interaction with CREBBP prevents its export to the cytoplasm.

Background

Members of the Interferon regulatory factor (IRF) family regulate gene expression critical to immune response, hemopoiesis, and proliferation. IRF-3 is a member of the IRF family, and is distinct from other family members. Its transcriptional activity is regulated solely by posttranslational modifications. It plays a crucial role in activation of innate immunity and inflammation in response to viral infection.

Database

Research Area

DATA IMAGES

Anti-IRF3 antibody [3F10] used in Western Blot (WB). GTX50058

GTX50058 WB Image

Cell lysates of HeLa(40ug) were resolved by SDS-PAGE, transferred to NC membrane and probed with anti-human IRF-3 (1:1,000). Proteins were visualized using a goat anti-mouse secondary antibody conjugated to HRP and an ECL detection system.

Anti-IRF3 antibody [3F10] used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX50058

GTX50058 ICC/IF Image

Immunofluorescence of human HeLa cells stained with IRF-3 (1:500) with Texas-Red and Hoechst 33342 (Blue) for nucleus staining.

REFERENCE

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REVIEW

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SDS
Sodium Azide.pdf
Package List Price ($)
$ 319