GeneTex
United States (US)

IRF3 antibody [4D4]

Overexpression
GTX84288_1010_ICCIF_18121411_238.jpg
GTX84288_4241_WB_18121411_575.jpg
GTX84288_343_FACS_18121411_213.jpg

Cat No. GTX84288

Host Mouse
Clonality Monoclonal
Clone Name 4D4
Isotype IgG2a
Application WB, ICC/IF, IHC-P, FACS
Reactivity Human
APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Dilution
WB 1:1000-1:2000
ICC/IF 1:100
IHC-P 1:50
FACS 1:100
Not tested in other applications.

Calculated MW

47 kDa. ( Note )

Specificity/Sensitivity

GTX84288 is specific for human IRF3.
PROPERTIES

Form

Liquid

Buffer

PBS pH 7.3, 1% BSA, 50% glycerol, 0.02% sodium azide

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

1mg/ml(Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

Full length human recombinant protein of human IRF3 (NP_001562) produced in HEK293T cell.

Purification

Purified by affinity chromatography
From tissue culture supernatant

Conjugation

Unconjugated

Note

For laboratory use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.
TARGET

Synonyms

IRF-3 antibody , interferon regulatory factor 3 antibody

Cellular Localization

Cytoplasm , Nucleus

Background

Mediates interferon-stimulated response element (ISRE) promoter activation. Functions as a molecular switch for antiviral activity. DsRNA generated during the course of an viral infection leads to IRF3 phosphorylation on the C-terminal serine/threonine cluster. This induces a conformational change, leading to its dimerization, nuclear localization and association with CREB binding protein (CREBBP) to form dsRNA-activated factor 1 (DRAF1), a complex which activates the transcription of genes under the control of ISRE. The complex binds to the IE and PRDIII regions on the IFN-alpha and IFN-beta promoters respectively. IRF-3 does not have any transcription activation domains.

Database

Research Area

DATA IMAGES
GTX84288_1010_ICCIF_18121411_238.jpg

GTX84288 ICC/IF Image

ICC/IF analysis of COS7 cells transiently transfected with IRF3 plasmid using GTX84288 IRF3 antibody [4D4].

GTX84288_4241_WB_18121411_575.jpg

GTX84288 WB Image

Overexpression

WB analysis of HEK293T cells transfected with IRF3 plasmid (Right) or empty vector (Left) for 48 hrs using GTX84288 IRF3 antibody [4D4].
Loading : 5 ug per lane

GTX84288_343_FACS_18121411_213.jpg

GTX84288 FACS Image

FACS analysis of HEK293T cells transfected with either IRF3 plasmid(Red) or empty vector control plasmid(Blue) using GTX84288 IRF3 antibody [4D4].

Package List Price ($)
$ 379