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IRF3 antibody

Comparison
Anti-IRF3 antibody used in Western Blot (WB). GTX133768

Cat. No. GTX133768

Reference 1
1

Host

 Rabbit

Clonality

 Polyclonal

Isotype

 IgG

Application

 WB

Reactivity

 Human
Package
100 μl ($399),
25 μl ($169)
See all IRF3 products

APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Recommended Dilution
WB 1:500-1:3000
Not tested in other applications.

Calculated MW

47 kDa. ( Note )

Positive Control

K562 , THP-1 , HL-60

Predict Reactivity

Rhesus Monkey(>80% identity)

PROPERTIES

Form

Liquid

Buffer

PBS, 20% Glycerol

Preservative

0.025% ProClin 300

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

0.56 mg/ml (Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

Full length human IRF3 recombinant protein.

Purification

Purified by antigen-affinity chromatography.

Conjugation

Unconjugated

RRID

AB_2887100

Note

For laboratory research use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

Purchasers shall not, and agree not to enable third parties to, analyze, copy, reverse engineer or otherwise attempt to determine the structure or sequence of the product.

TARGET

Synonyms

interferon regulatory factor 3 , IIAE7

Cellular Localization

Cytoplasm , Nucleus

Background

IRF3 encodes interferon regulatory factor 3, a member of the interferon regulatory transcription factor (IRF) family. IRF3 is found in an inactive cytoplasmic form that upon serine/threonine phosphorylation forms a complex with CREBBP. This complex translocates to the nucleus and activates the transcription of interferons alpha and beta, as well as other interferon-induced genes. [provided by RefSeq]

Database

Research Area

DATA IMAGES

Anti-IRF3 antibody used in Western Blot (WB). GTX133768

GTX133768 WB Image

Comparison

Various whole cell extracts (30 μg) were separated by 10% SDS-PAGE, and the membranes were blotted with IRF3 antibody (GTX133768) diluted at 1:1000 and competitor's antibody (sc-9082) diluted at 1:100. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.
*The competitor is not affiliated with GeneTex and does not endorse this product.

REFERENCE

Application Reference

WC Chiou et al. Biomed Pharmacother 2023; 170 : 116077 Ergosterol peroxide blocks HDV infection as a novel entry inhibitor by targeting human NTCP receptor
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REVIEW

IRF3 antibody Cat. No. GTX133768

Rating
( Average 5 based on 1 users reviews)
Application
Western Blot(WB)
( Average 5 based on 1 users reviews)
Anti-IRF3 antibody used in Western Blot (WB). GTX133768
Date : Anonymous submitted on 16-Sep-2021
Score :
Application Tested : WB
Sample Species : Hu
Sample : human breast cancer cell lines
Amount used : 30μg
Blocking : 5% non-fat milk in TBST, RT, 0.5Hr
Primary Antibody : 1:1000, 4°C, > 12Hr
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SDS
PBS.pdf
Glycerol.pdf
Proclin.pdf
Package List Price ($)
$ 399
$ 169