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IRF3 antibody

Anti-IRF3 antibody used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX31695
Anti-IRF3 antibody used in Western Blot (WB). GTX31695
Anti-IRF3 antibody used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX31695

Cat. No. GTX31695

Host

 Rabbit

Clonality

 Polyclonal

Isotype

 IgG

Application

 WB, ICC/IF, ELISA

Reactivity

 Human, Rat
Package
100 μg ($399)
See all IRF3 products

APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Recommended Dilution
WB 1 μg/mL
ICC/IF 2 μg/mL
ELISA Assay dependent
Not tested in other applications.

Calculated MW

47 kDa. ( Note )

Positive Control

Ramos Cell Lysate(GTX23955) , Rat Kidney Tissue Lysate(GTX27944) , Human Kidney Tissue Lysate(GTX29995)

PROPERTIES

Form

Liquid

Buffer

PBS

Preservative

0.02% Sodium azide

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

1 mg/ml (Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

IRF3 antibody antibody was raised against a peptide corresponding to 14 amino acids near the center of human IRF3.The immunogen is located within amino acids 150 - 200 of IRF3.

Purification

Purified by antigen-affinity chromatography

Conjugation

Unconjugated

Note

For laboratory research use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

Purchasers shall not, and agree not to enable third parties to, analyze, copy, reverse engineer or otherwise attempt to determine the structure or sequence of the product.

TARGET

Synonyms

interferon regulatory factor 3 , IIAE7

Cellular Localization

Cytoplasm,Nucleus

Background

This gene encodes a member of the interferon regulatory transcription factor (IRF) family. The encoded protein is found in an inactive cytoplasmic form that upon serine/threonine phosphorylation forms a complex with CREBBP. This complex translocates to the nucleus and activates the transcription of interferons alpha and beta, as well as other interferon-induced genes. Alternatively spliced transcript variants encoding multiple isoforms have been observed for this gene. [provided by RefSeq, Nov 2011]

Database

Research Area

DATA IMAGES

Anti-IRF3 antibody used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX31695

GTX31695 ICC/IF Image

ICC/IF analysis of Ramos cells using GTX31695 IRF3 antibody.
Working concentration : 2 μg/ml

Anti-IRF3 antibody used in Western Blot (WB). GTX31695

GTX31695 WB Image

WB analysis of (A) human kidney and (B) rat kidney lysate using GTX31695 IRF3 antibody.
Working concentration : 1 μg/ml

Anti-IRF3 antibody used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX31695

GTX31695 ICC/IF Image

ICC/IF analysis of Ramos cells using GTX31695 IRF3 antibody.
Working concentration : 2 μg/ml

REFERENCE

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REVIEW

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SDS
PBS.pdf
Sodium Azide.pdf
Package List Price ($)
$ 399