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IRF3 antibody

Anti-IRF3 antibody used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX54366
Anti-IRF3 antibody used in IHC (Paraffin sections) (IHC-P). GTX54366
Anti-IRF3 antibody used in IHC (Paraffin sections) (IHC-P). GTX54366
Anti-IRF3 antibody used in Western Blot (WB). GTX54366
Anti-IRF3 antibody used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX54366
Anti-IRF3 antibody used in IHC (Paraffin sections) (IHC-P). GTX54366

Cat. No. GTX54366

Reference 1

Host

 Rabbit

Clonality

 Polyclonal

Isotype

 IgG

Application

 WB, ICC/IF, IHC-P

Reactivity

 Human, Mouse, Rat
Package
100 μl ($399)
See all IRF3 products

APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Recommended Dilution
WB 1:500 - 1:2000
ICC/IF 1:50 - 1:200
IHC-P 1:50 - 1:200
Not tested in other applications.

Calculated MW

47 kDa. ( Note )

PROPERTIES

Form

Liquid

Buffer

PBS, 50% Glycerol

Preservative

0.02% Sodium azide

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

Batch dependent (Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

A synthetic peptide of human IRF3

Purification

Purified by affinity chromatography

Conjugation

Unconjugated

Note

For laboratory research use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

Purchasers shall not, and agree not to enable third parties to, analyze, copy, reverse engineer or otherwise attempt to determine the structure or sequence of the product.

TARGET

Synonyms

interferon regulatory factor 3 , IIAE7

Cellular Localization

Cytoplasm,Nucleus

Background

This gene encodes a member of the interferon regulatory transcription factor (IRF) family. The encoded protein is found in an inactive cytoplasmic form that upon serine/threonine phosphorylation forms a complex with CREBBP. This complex translocates to the nucleus and activates the transcription of interferons alpha and beta, as well as other interferon-induced genes. Alternatively spliced transcript variants encoding multiple isoforms have been observed for this gene. [provided by RefSeq, Nov 2011]

Database

Research Area

DATA IMAGES

Anti-IRF3 antibody used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX54366

GTX54366 ICC/IF Image

ICC/IF analysis of U2OS cells using GTX54366 IRF3 antibody.
Blue : DAPI
Dilution : 1:100

Anti-IRF3 antibody used in IHC (Paraffin sections) (IHC-P). GTX54366

GTX54366 IHC-P Image

IHC-P analysis of rat heart tissue using GTX54366 IRF3 antibody.
Dilution : 1:100

Anti-IRF3 antibody used in IHC (Paraffin sections) (IHC-P). GTX54366

GTX54366 IHC-P Image

IHC-P analysis of mouse kidney tissue using GTX54366 IRF3 antibody.
Dilution : 1:100

Anti-IRF3 antibody used in Western Blot (WB). GTX54366

GTX54366 WB Image

WB analysis of various sample lysates using GTX54366 IRF3 antibody.
Dilution : 1:1000
Loading : 25μg per lane

Anti-IRF3 antibody used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX54366

GTX54366 ICC/IF Image

ICC/IF analysis of HeLa cells using GTX54366 IRF3 antibody.
Blue : DAPI
Dilution : 1:100

Anti-IRF3 antibody used in IHC (Paraffin sections) (IHC-P). GTX54366

GTX54366 IHC-P Image

IHC-P analysis of human colon tissue using GTX54366 IRF3 antibody.
Dilution : 1:100

REFERENCE

Application Reference

Meiling Yan et al. Cell Death Discov 2022; 8 (1) : 258 Mitochondrial damage and activation of the cytosolic DNA sensor cGAS-STING pathway lead to cardiac pyroptosis and hypertrophy in diabetic cardiomyopathy mice.
Application : WB
Reactivity : Mouse
Submit a Reference

REVIEW

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SDS
PBS.pdf
Glycerol.pdf
Sodium Azide.pdf
Package List Price ($)
$ 399