GeneTex
United States (US)

IRF3 antibody

KO/KD
GTX64358_20180530_IHC-P_18121411_313.jpg
GTX64358_20180530_IHC-P_1_18121411_228.jpg
GTX64358_20180530_ChIPassay_18121411_129.jpg
GTX64358_20180530_WB_18121411_365.jpg
GTX64358_20180822_WB_18121411_415.jpg

Cat No. GTX64358

Host Rabbit
Clonality Polyclonal
Isotype IgG
Application WB, IHC-P, ChIP assay
Reactivity Human
APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Dilution
WB 1:500 - 1:2000
IHC-P 1:50 - 1:200
ChIP assay 1:20 - 1:100
Not tested in other applications.

Calculated MW

47 kDa. ( Note )
PROPERTIES

Form

Liquid

Buffer

PBS pH7.3, 0.02% sodium azide, 50% glycerol.

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Antigen Species

Human

Immunogen

Recombinant funsion protein containing a sequence corresponding to amino acids 1-260 of human IRF3 (NP_001184051.1).

Purification

Purified by affinity chromatography

Conjugation

Unconjugated

Note

For In vitro laboratory use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.
TARGET

Background

This gene encodes a member of the interferon regulatory transcription factor (IRF) family. The encoded protein is found in an inactive cytoplasmic form that upon serine/threonine phosphorylation forms a complex with CREBBP. This complex translocates to the nucleus and activates the transcription of interferons alpha and beta, as well as other interferon-induced genes. Alternatively spliced transcript variants encoding multiple isoforms have been observed for this gene. [provided by RefSeq, Nov 2011]

Database

Research Area

DATA IMAGES
GTX64358_20180530_IHC-P_18121411_313.jpg

GTX64358 IHC-P Image

IHC-P analysis of human esophageal cancer tissue using GTX64358 IRF3 antibody.
Dilution : 1:100

GTX64358_20180530_IHC-P_1_18121411_228.jpg

GTX64358 IHC-P Image

IHC-P analysis of human lung cancer tissue using GTX64358 IRF3 antibody.
Dilution : 1:100

GTX64358_20180530_ChIPassay_18121411_129.jpg

GTX64358 ChIP assay Image

ChIP analysis of HCT116 cells using GTX64358 IRF3 antibody.The amount of immunoprecipitated DNA was checked by quantitative PCR. Histogram was constructed by the ratios of the immunoprecipitated DNA to the input.

GTX64358_20180530_WB_18121411_365.jpg

GTX64358 WB Image

WB analysis of various samples using GTX64358 IRF3 antibody.
Dilution : 1:3000
Loading : 25μg per lane

GTX64358_20180822_WB_18121411_415.jpg

GTX64358 WB Image

KO/KD

WB analysis of wild type and IRF3 knockout cell lysate using GTX64358 IRF3 antibody.
Dilution : 1:3000
Loading : 25μg per lane

Package List Price ($)
$ 339