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IRF3 antibody

KO/KD
Anti-IRF3 antibody used in Western Blot (WB). GTX64358
Anti-IRF3 antibody used in IHC (Paraffin sections) (IHC-P). GTX64358
Anti-IRF3 antibody used in IHC (Paraffin sections) (IHC-P). GTX64358
Anti-IRF3 antibody used in ChIP assay (ChIP assay). GTX64358
Anti-IRF3 antibody used in Western Blot (WB). GTX64358

Cat. No. GTX64358

Host

 Rabbit

Clonality

 Polyclonal

Isotype

 IgG

Application

 WB, IHC-P, ChIP assay

Reactivity

 Human
Package
100 μl ($399)
See all IRF3 products

APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Recommended Dilution
WB 1:500 - 1:2000
IHC-P 1:50 - 1:200
ChIP assay 1:20 - 1:100
Not tested in other applications.

Calculated MW

47 kDa. ( Note )

PROPERTIES

Form

Liquid

Buffer

PBS, 50% Glycerol

Preservative

0.02% Sodium azide

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

Batch dependent (Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

Recombinant fusion protein containing a sequence corresponding to amino acids 1-260 of human IRF3 (NP_001184051.1).

Purification

Purified by affinity chromatography

Conjugation

Unconjugated

Note

For laboratory research use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

Purchasers shall not, and agree not to enable third parties to, analyze, copy, reverse engineer or otherwise attempt to determine the structure or sequence of the product.

TARGET

Synonyms

interferon regulatory factor 3 , IIAE7

Cellular Localization

Cytoplasm,Nucleus

Background

This gene encodes a member of the interferon regulatory transcription factor (IRF) family. The encoded protein is found in an inactive cytoplasmic form that upon serine/threonine phosphorylation forms a complex with CREBBP. This complex translocates to the nucleus and activates the transcription of interferons alpha and beta, as well as other interferon-induced genes. Alternatively spliced transcript variants encoding multiple isoforms have been observed for this gene. [provided by RefSeq, Nov 2011]

Database

Research Area

DATA IMAGES

Anti-IRF3 antibody used in Western Blot (WB). GTX64358

GTX64358 WB Image

KO/KD

WB analysis of normal (control) and knockout (KO) HeLa cell lysate using GTX64358 IRF3 antibody.
Dilution : 1:1000
Loading : 25μg per lane

Anti-IRF3 antibody used in IHC (Paraffin sections) (IHC-P). GTX64358

GTX64358 IHC-P Image

IHC-P analysis of human lung cancer tissue using GTX64358 IRF3 antibody.
Dilution : 1:100

Anti-IRF3 antibody used in IHC (Paraffin sections) (IHC-P). GTX64358

GTX64358 IHC-P Image

IHC-P analysis of human esophageal cancer tissue using GTX64358 IRF3 antibody.
Dilution : 1:100

Anti-IRF3 antibody used in ChIP assay (ChIP assay). GTX64358

GTX64358 ChIP assay Image

ChIP analysis of HCT116 cell lysate using GTX64358 IRF3 antibody. The amount of immunoprecipitated DNA was checked by quantitative PCR. Histogram was constructed by the ratios of the immunoprecipitated DNA to the input.

Anti-IRF3 antibody used in Western Blot (WB). GTX64358

GTX64358 WB Image

WB analysis of various sample lysates using GTX64358 IRF3 antibody.
Dilution : 1:3000
Loading : 25μg per lane

REFERENCE

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REVIEW

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SDS
PBS.pdf
Glycerol.pdf
Sodium Azide.pdf
Package List Price ($)
$ 399