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IRF3 (phospho Ser396) antibody

Anti-IRF3 (phospho Ser396) antibody used in Western Blot (WB). GTX02883
Anti-IRF3 (phospho Ser396) antibody used in IHC (Paraffin sections) (IHC-P). GTX02883
Anti-IRF3 (phospho Ser396) antibody used in ELISA (ELISA). GTX02883
Anti-IRF3 (phospho Ser396) antibody used in IHC (Paraffin sections) (IHC-P). GTX02883
Anti-IRF3 (phospho Ser396) antibody used in IHC (Paraffin sections) (IHC-P). GTX02883
Anti-IRF3 (phospho Ser396) antibody used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX02883

Cat. No. GTX02883

Host

 Rabbit

Clonality

 Polyclonal

Isotype

 IgG

Application

 WB, ICC/IF, IHC-P, ELISA

Reactivity

 Human, Mouse, Rat
Package
100 μl ($399)
See all IRF3 products

APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Recommended Dilution
WB 1:500-1:2000
ICC/IF 1:100-1:500
IHC-P 1:50-1:200
ELISA Assay dependent
Not tested in other applications.

Calculated MW

47 kDa. ( Note )

Predict Reactivity

Sheep, Bovine, Pig, Horse(>80% identity)

PROPERTIES

Form

Liquid

Buffer

PBS, 150mM NaCl, 50% Glycerol

Preservative

0.02% Sodium azide

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

1 mg/ml (Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

A synthesized peptide derived from human IRF3 around the phosphorylation site of Ser396.

Purification

Purified by affinity purification via sequential chromatography on phospho-peptide and non-phospho-peptide affinity columns
From serum

Conjugation

Unconjugated

Note

For laboratory research use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

Purchasers shall not, and agree not to enable third parties to, analyze, copy, reverse engineer or otherwise attempt to determine the structure or sequence of the product.

TARGET

Synonyms

interferon regulatory factor 3 , IIAE7

Cellular Localization

Cytoplasm , Nucleus

Background

This gene encodes a member of the interferon regulatory transcription factor (IRF) family. The encoded protein is found in an inactive cytoplasmic form that upon serine/threonine phosphorylation forms a complex with CREBBP. This complex translocates to the nucleus and activates the transcription of interferons alpha and beta, as well as other interferon-induced genes. Alternatively spliced transcript variants encoding multiple isoforms have been observed for this gene. [provided by RefSeq, Nov 2011]

Database

Research Area

DATA IMAGES

Anti-IRF3 (phospho Ser396) antibody used in Western Blot (WB). GTX02883

GTX02883 WB Image

WB analysis of plpC-transfected HT29 cell lysate using GTX02883 IRF3 (phospho Ser396) antibody.
N peptide : Non-phospho peptide
P peptide : Phospho peptide

Anti-IRF3 (phospho Ser396) antibody used in IHC (Paraffin sections) (IHC-P). GTX02883

GTX02883 IHC-P Image

IHC-P analysis of rat heart tissue using GTX02883 IRF3 (phospho Ser396) antibody.
Antigen retrieval : Heat mediated antigen retrieval step in citrate buffer was performed
Dilution : 1:100

Anti-IRF3 (phospho Ser396) antibody used in ELISA (ELISA). GTX02883

GTX02883 ELISA Image

ELISA analysis of phospho- and non phosho- immunogen peptide using GTX02883 IRF3 (phospho Ser396) antibody.
P-peptide : phospho-peptide
N-peptide : non-phospho-peptide
Peptides concentration : 1μg/ml

Anti-IRF3 (phospho Ser396) antibody used in IHC (Paraffin sections) (IHC-P). GTX02883

GTX02883 IHC-P Image

IHC-P analysis of mouse heart tissue using GTX02883 IRF3 (phospho Ser396) antibody.
Antigen retrieval : Heat mediated antigen retrieval step in citrate buffer was performed
Dilution : 1:100

Anti-IRF3 (phospho Ser396) antibody used in IHC (Paraffin sections) (IHC-P). GTX02883

GTX02883 IHC-P Image

IHC-P analysis of mouse thymus tissue using GTX02883 IRF3 (phospho Ser396) antibody.
Antigen retrieval : Heat mediated antigen retrieval step in citrate buffer was performed
Dilution : 1:100

Anti-IRF3 (phospho Ser396) antibody used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX02883

GTX02883 ICC/IF Image

ICC/IF analysis of PFA-fixed HepG2 cells (30min of 4μM Forskolin treatment) using GTX02883 IRF3 (phospho Ser396) antibody.
Red : Primary antibody
Green : Beta tubulin
Blue : DAPI
Permeabilization : 0.1% Triton X-100

REFERENCE

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REVIEW

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Package List Price ($)
$ 399