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IRF3 (phospho Ser396) antibody

Orthogonal
Anti-IRF3 (phospho Ser396) antibody used in Western Blot (WB). GTX134360
Anti-IRF3 (phospho Ser396) antibody used in IHC (Paraffin sections) (IHC-P). GTX134360

Cat. No. GTX134360

Host

 Rabbit

Clonality

 Polyclonal

Isotype

 IgG

Application

 WB, IHC-P

Reactivity

 Human, Mouse
Package
100 μl ($399),
25 μl ($169)
See all IRF3 products

APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Recommended Dilution
WB 1:500
IHC-P Assay dependent
Not tested in other applications.

Calculated MW

47 kDa. ( Note )

Predict Reactivity

Rat, Bovine, Pig, Rhesus Monkey(>80% identity)

PROPERTIES

Form

Liquid

Buffer

PBS, 1% BSA, 20% Glycerol

Preservative

0.025% ProClin 300

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

1.54 mg/ml (Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

Carrier-protein conjugated synthetic peptide surrounding phospho Ser396 of human IRF3. The exact sequence is proprietary.

Purification

Purified by antigen-affinity chromatography.

Conjugation

Unconjugated

RRID

AB_2887258

Note

For laboratory research use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

Purchasers shall not, and agree not to enable third parties to, analyze, copy, reverse engineer or otherwise attempt to determine the structure or sequence of the product.

TARGET

Synonyms

interferon regulatory factor 3 , IIAE7

Cellular Localization

Cytoplasm,Nucleus

Background

This gene encodes a member of the interferon regulatory transcription factor (IRF) family. The encoded protein is found in an inactive cytoplasmic form that upon serine/threonine phosphorylation forms a complex with CREBBP. This complex translocates to the nucleus and activates the transcription of interferons alpha and beta, as well as other interferon-induced genes. Alternatively spliced transcript variants encoding multiple isoforms have been observed for this gene. [provided by RefSeq, Nov 2011]

Database

Research Area

DATA IMAGES

Anti-IRF3 (phospho Ser396) antibody used in Western Blot (WB). GTX134360

GTX134360 WB Image

Orthogonal

Untreated (–) and treated (+) THP-1 whole cell extracts (30 μg) were separated by 10% SDS-PAGE, and the membrane was blotted with IRF3 (phospho Ser396) antibody (GTX134360) diluted at 1:500. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody, and the signal was developed with Trident ECL plus-Enhanced.

Anti-IRF3 (phospho Ser396) antibody used in IHC (Paraffin sections) (IHC-P). GTX134360

GTX134360 IHC-P Image

IRF3 (phospho Ser396) antibody detects IRF3 (phospho Ser396) protein at nucleus by immunohistochemical analysis.
Sample: Paraffin-embedded mouse spleen.
IRF3 (phospho Ser396) stained by IRF3 (phospho Ser396) antibody (GTX134360) diluted at 1:500.
Antigen Retrieval: Citrate buffer, pH 6.0, 15 min

REFERENCE

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REVIEW

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SDS
PBS.pdf
Glycerol.pdf
Proclin.pdf
Package List Price ($)
$ 399
$ 169
Currently in production