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IRF3 (phospho Ser396) antibody

Anti-IRF3 (phospho Ser396) antibody used in Western Blot (WB). GTX86691

Cat. No. GTX86691

Reference 11

Host

 Rabbit

Clonality

 Polyclonal

Isotype

 IgG

Application

 WB

Reactivity

 Human, Mouse
Package
100 μl ($419)
See all IRF3 products

APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Recommended Dilution
WB 1:500~1:1000
Not tested in other applications.

Calculated MW

47 kDa. ( Note )

PROPERTIES

Form

Liquid

Buffer

PBS, 150mM NaCl, 50% Glycerol

Preservative

0.02% Sodium azide

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

Batch dependent (Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

The antiserum was produced against synthesized peptide derived from human IRF3 (362-411).

Purification

Purified by antigen-affinity chromatography. Non-phospho peptide was removed by chromatography using corresponding non-phospho peptide.
From serum

Conjugation

Unconjugated

Note

For laboratory use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

TARGET

Synonyms

IIAE7 , IRF3 , interferon regulatory factor 3

Cellular Localization

Cytoplasm,Nucleus

Background

This gene encodes a member of the interferon regulatory transcription factor (IRF) family. The encoded protein is found in an inactive cytoplasmic form that upon serine/threonine phosphorylation forms a complex with CREBBP. This complex translocates to the nucleus and activates the transcription of interferons alpha and beta, as well as other interferon-induced genes. Alternatively spliced transcript variants encoding multiple isoforms have been observed for this gene. [provided by RefSeq, Nov 2011]

Database

Research Area

DATA IMAGES

Anti-IRF3 (phospho Ser396) antibody used in Western Blot (WB). GTX86691

GTX86691 WB Image

WB analysis of Jurkat cells treated with EGF 200ng/ml (30mins) lysate using GTX86691 IRF3 (phospho Ser396) antibody. The lane on the right is blocked with the synthesized peptide.

REFERENCE

Application Reference

C Li et al. Chinese Herbal Medicines 2025; Salvia miltiorrhiza water extract ameliorated cGAS-STING-mediated acute liver injury by blocking interaction between STING and TBK1
C Zheng et al. Mol Med 2024; 30 (1) : 160 Pentagalloylglucose alleviates acetaminophen-induced acute liver injury by modulating inflammation via cGAS-STING pathway
Yang H et al. Front Pharmacol 2024; 15 : 1369563 Schisandrin C enhances type I IFN response activation to reduce tumor growth and sensitize chemotherapy through antitumor immunity.
C Li et al. Chin Med 2024; 19 (1) : 107 Total tanshinones ameliorates cGAS-STING-mediated inflammatory and autoimmune diseases by affecting STING-IRF3 binding
Y Xiu et al. Chin J Nat Med 2024; 22 (５) : 402-415 Total glucosides of paeony alleviates cGAS-STING-mediated diseases by blocking the STING-IRF3 interaction
Application : WB
Reactivity : Mouse
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REVIEW

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Application Reference
C Li et al. Chinese Herbal Medicines 2025; Salvia miltiorrhiza water extract ameliorated cGAS-STING-mediated acute liver injury by blocking interaction between STING and TBK1
C Zheng et al. Mol Med 2024; 30 (1):160 Pentagalloylglucose alleviates acetaminophen-induced acute liver injury by modulating inflammation via cGAS-STING pathway
Yang H et al. Front Pharmacol 2024; 15:1369563 Schisandrin C enhances type I IFN response activation to reduce tumor growth and sensitize chemotherapy through antitumor immunity.
C Li et al. Chin Med 2024; 19 (1):107 Total tanshinones ameliorates cGAS-STING-mediated inflammatory and autoimmune diseases by affecting STING-IRF3 binding
Y Xiu et al. Chin J Nat Med 2024; 22 (５):402-415 Total glucosides of paeony alleviates cGAS-STING-mediated diseases by blocking the STING-IRF3 interaction
Application : WB
Reactivity : Mouse
Siwen Hui et al. Front Pharmacol 2024; 15:1374179 Glycyrrhiza uralensis polysaccharides ameliorates cecal ligation and puncture-induced sepsis by inhibiting the cGAS-STING signaling pathway
Application : WB
Reactivity : Mouse
J Li et al. Chin Herb Med 2024; Amplifying protection against acute lung injury: targeting both inflammasome and cGAS-STING pathway by Lonicerae Japonicae Flos-Forsythiae Fructus drug pair
W Shi et al. Phytomedicine 2024; 28:155404 Compound Danshen Dripping Pill effectively alleviates cGAS-STING-triggered diseases by disrupting STING-TBK1 interaction
Application : WB
Reactivity : Mouse
Y Wang et al. Immunology 2024; Flavonoid extracted from Epimedium attenuate cGAS-STING-mediated diseases by targeting the formation of functional STING signalosome
Application : WB
Reactivity : Mouse
Luo W et al. Int Immunopharmacol 2024; 128:111550 LicochalconeB inhibits cGAS-STING signaling pathway and prevents autoimmunity diseases
J Wen et al. Mol Med 2023; 29 (1):167 Glabridin improves autoimmune disease in Trex1-deficient mice by reducing type I interferon production
SDS
PBS.pdf
Glycerol.pdf
Sodium Azide.pdf
Package List Price ($)
$ 419