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IRF4 / MUM1 antibody

Anti-IRF4 / MUM1 antibody used in Immunoprecipitation (IP). GTX129135
Anti-IRF4 / MUM1 antibody used in Western Blot (WB). GTX129135

Cat No. GTX129135

Host

Rabbit

Clonality

Polyclonal

Isotype

IgG

Application

WB, IP

Reactivity

Human
Package
100 μl ($329),
25 μl ($169)

APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Dilution
WB 1:500-1:3000
IP 1:100-1:500
Not tested in other applications.

Calculated MW

52 kDa. ( Note )

Positive Control

NCI-H929

Predict Reactivity

Mouse, Rat, Bovine, Chicken, Pig, Rhesus Monkey(>80% identity)

PROPERTIES

Form

Liquid

Buffer

1XPBS, 20% Glycerol (pH7). 0.01% Thimerosal was added as a preservative.

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

1 mg/ml (Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

Recombinant protein encompassing a sequence within the center region of human IRF4 / MUM1. The exact sequence is proprietary.

Purification

Purified by antigen-affinity chromatography.

Conjugation

Unconjugated

RRID

AB_2885906

Note

For laboratory use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

TARGET

Synonyms

interferon regulatory factor 4 , LSIRF , MUM1 , NF-EM5 , SHEP8

Cellular Localization

Nucleus

Background

The protein encoded by this gene belongs to the IRF (interferon regulatory factor) family of transcription factors, characterized by an unique tryptophan pentad repeat DNA-binding domain. The IRFs are important in the regulation of interferons in response to infection by virus, and in the regulation of interferon-inducible genes. This family member is lymphocyte specific and negatively regulates Toll-like-receptor (TLR) signaling that is central to the activation of innate and adaptive immune systems. A chromosomal translocation involving this gene and the IgH locus, t(6;14)(p25;q32), may be a cause of multiple myeloma. Alternatively spliced transcript variants have been found for this gene. [provided by RefSeq]

Database

Research Area

DATA IMAGES

Anti-IRF4 / MUM1 antibody used in Immunoprecipitation (IP). GTX129135

GTX129135 IP Image

Immunoprecipitation of IRF4 / MUM1 protein from NCI-H929 whole cell extracts using 5 μg of IRF4 / MUM1 antibody (GTX129135).
Western blot analysis was performed using IRF4 / MUM1 antibody (GTX129135) diluted at 1:500.
EasyBlot anti-Rabbit IgG (GTX221666-01) was used as a secondary reagent.

Anti-IRF4 / MUM1 antibody used in Western Blot (WB). GTX129135

GTX129135 WB Image

IRF4 / MUM1 antibody detects IRF4 / MUM1 protein by Western blot analysis.
A. 30 μg NCI-H929 whole cell lysate/extract
10 % SDS-PAGE
IRF4 / MUM1 antibody (GTX129135) dilution: 1:1000

REFERENCE

REVIEW

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SDS
PBS.pdf
Glycerol.pdf
Thimerosal.pdf