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IRF4 / MUM1 antibody

Orthogonal
Anti-IRF4 / MUM1 antibody used in Western Blot (WB). GTX129135
Anti-IRF4 / MUM1 antibody used in Immunoprecipitation (IP). GTX129135

Cat. No. GTX129135

Reference 1

Host

 Rabbit

Clonality

 Polyclonal

Isotype

 IgG

Application

 WB, IP

Reactivity

 Human
Package
100 μl ($399),
25 μl ($169)
See all IRF4 / MUM1 products

APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Recommended Dilution
WB 1:500-1:3000
IP 1:100-1:500
Not tested in other applications.

Calculated MW

52 kDa. ( Note )

Positive Control

NCI-H929

Predict Reactivity

Mouse, Rat, Bovine, Chicken, Pig, Rhesus Monkey(>80% identity)

PROPERTIES

Form

Liquid

Buffer

PBS, 20% Glycerol

Preservative

0.01% Thimerosal

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

1 mg/ml (Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

Recombinant protein encompassing a sequence within the center region of human IRF4 / MUM1. The exact sequence is proprietary.

Purification

Purified by antigen-affinity chromatography.

Conjugation

Unconjugated

RRID

AB_2885906

Note

For laboratory research use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

Purchasers shall not, and agree not to enable third parties to, analyze, copy, reverse engineer or otherwise attempt to determine the structure or sequence of the product.

TARGET

Synonyms

interferon regulatory factor 4 , LSIRF , MUM1 , NF-EM5 , SHEP8

Cellular Localization

Nucleus

Background

The protein encoded by this gene belongs to the IRF (interferon regulatory factor) family of transcription factors, characterized by an unique tryptophan pentad repeat DNA-binding domain. The IRFs are important in the regulation of interferons in response to infection by virus, and in the regulation of interferon-inducible genes. This family member is lymphocyte specific and negatively regulates Toll-like-receptor (TLR) signaling that is central to the activation of innate and adaptive immune systems. A chromosomal translocation involving this gene and the IgH locus, t(6;14)(p25;q32), may be a cause of multiple myeloma. Alternatively spliced transcript variants have been found for this gene. [provided by RefSeq]

Database

Research Area

DATA IMAGES

Anti-IRF4 / MUM1 antibody used in Western Blot (WB). GTX129135

GTX129135 WB Image

Orthogonal

Various whole cell extracts (30 μg) were separated by 10% SDS-PAGE, and the membrane was blotted with IRF4 / MUM1 antibody (GTX129135) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody. Corresponding RNA expression data for the same cell lines are based on Human Protein Atlas program.

Anti-IRF4 / MUM1 antibody used in Immunoprecipitation (IP). GTX129135

GTX129135 IP Image

Immunoprecipitation of IRF4 / MUM1 protein from NCI-H929 whole cell extracts using 5 μg of IRF4 / MUM1 antibody (GTX129135).
Western blot analysis was performed using IRF4 / MUM1 antibody (GTX129135) diluted at 1:500.
EasyBlot anti-Rabbit IgG (GTX221666-01) was used as a secondary reagent.

REFERENCE

Application Reference

Fucci C et al. Cell Rep 2020; 32 (12) : 108162 The Interaction of the Tumor Suppressor FAM46C with p62 and FNDC3 Proteins Integrates Protein and Secretory Homeostasis.
Application : WB
Reactivity : Human
Submit a Reference

REVIEW

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SDS
PBS.pdf
Glycerol.pdf
Thimerosal.pdf
Package List Price ($)
$ 399
$ 169