GeneTex
United States (US)

IRF4 antibody

GTX31438_173_ICC-IF_20180221_18121410_578.jpg
GTX31438_2634_WB_20180221_18121410_528.jpg
GTX31438_172_ICC-IF_20180221_18121410_738.jpg

Cat No. GTX31438

Host Rabbit
Clonality Polyclonal
Isotype IgG
Application WB, ICC/IF, ELISA
Reactivity Human, Mouse, Rat
APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Dilution
WB 0.5 μg/mL
ICC/IF 10 μg/mL
ELISA Assay dependent
Not tested in other applications.

Calculated MW

52 kDa. ( Note )

Positive Control

Ramos Cell Lysate(GTX23955) , Jurkat Cell Slide(GTX25533)

Specificity/Sensitivity

IRF4 antibody is predicted to not cross-react with other IRF protein family members. IRF4 often migrates at a higher than predicted molecular weight in SDS-PAGE.
PROPERTIES

Form

Liquid

Buffer

PBS with 0.02% sodium azide.

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. Store at 4ºC.

Concentration

1 mg/ml (Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

IRF4 antibody was raised against a 16 amino acid synthetic peptide near the carboxy terminus of human IRF4.The immunogen is located within the last 50 amino acids of IRF4.

Purification

Purified by antigen-affinity chromatography

Conjugation

Unconjugated

Note

For laboratory use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.
TARGET

Synonyms

Interferon Regulatory Factor 4,Lsirf,Mum1,Nf-Em5,Shep8,Irf4

Background

The protein encoded by this gene belongs to the IRF (interferon regulatory factor) family of transcription factors, characterized by an unique tryptophan pentad repeat DNA-binding domain. The IRFs are important in the regulation of interferons in response to infection by virus, and in the regulation of interferon-inducible genes. This family member is lymphocyte specific and negatively regulates Toll-like-receptor (TLR) signaling that is central to the activation of innate and adaptive immune systems. A chromosomal translocation involving this gene and the IgH locus, t(6;14)(p25;q32), may be a cause of multiple myeloma. Alternatively spliced transcript variants have been found for this gene. [provided by RefSeq, Aug 2010]

Database

Research Area

DATA IMAGES
GTX31438_173_ICC-IF_20180221_18121410_578.jpg

GTX31438 ICC/IF Image

ICC/IF analysis of Jurkat cells using GTX31438 IRF4 antibody.
Working concentration : 20 μg/ml

GTX31438_2634_WB_20180221_18121410_528.jpg

GTX31438 WB Image

WB analysis of Ramos cell lysate using GTX31438 IRF4 antibody.
Working concentration : 0.5 μg/ml

GTX31438_172_ICC-IF_20180221_18121410_738.jpg

GTX31438 ICC/IF Image

ICC/IF analysis of Jurkat cells using GTX31438 IRF4 antibody.
Working concentration : 10 μg/ml