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IRF5 antibody [N1C1]

Anti-IRF5 antibody [N1C1] used in Western Blot (WB). GTX113477
Anti-IRF5 antibody [N1C1] used in Western Blot (WB). GTX113477
Anti-IRF5 antibody [N1C1] used in Western Blot (WB). GTX113477

Cat. No. GTX113477

Reference 2

Host

 Rabbit

Clonality

 Polyclonal

Isotype

 IgG

Application

 WB, IHC-P

Reactivity

 Human, Mouse
Package
100 μl ($399),
25 μl ($169)
See all IRF5 products

APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Recommended Dilution
WB 1:500-1:3000
IHC-P Assay dependent
Not tested in other applications.

Calculated MW

56 kDa. ( Note )

Positive Control

HepG2 , Neuro 2A , C8D30 , NIH-3T3 , Raw264.7 , C2C12 , mouse spleen

PROPERTIES

Form

Liquid

Buffer

PBS, 1% BSA, 20% Glycerol

Preservative

0.01% Thimerosal

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

1 mg/ml (Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

Recombinant protein encompassing a sequence within the center region of human IRF5. The exact sequence is proprietary.

Purification

Purified by antigen-affinity chromatography.

Conjugation

Unconjugated

RRID

AB_11169031

Note

For laboratory research use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

Purchasers shall not, and agree not to enable third parties to, analyze, copy, reverse engineer or otherwise attempt to determine the structure or sequence of the product.

TARGET

Synonyms

interferon regulatory factor 5 , SLEB10

Cellular Localization

Nucleus

Background

This gene encodes a member of the interferon regulatory factor (IRF) family, a group of transcription factors with diverse roles, including virus-mediated activation of interferon, and modulation of cell growth, differentiation, apoptosis, and immune system activity. Members of the IRF family are characterized by a conserved N-terminal DNA-binding domain containing tryptophan (W) repeats. Alternative splice variants encoding different isoforms exist. [provided by RefSeq]

Database

Research Area

DATA IMAGES

Anti-IRF5 antibody [N1C1] used in Western Blot (WB). GTX113477

GTX113477 WB Image

Various whole cell extracts (30 μg) were separated by 10% SDS-PAGE, and the membrane was blotted with IRF5 antibody [N1C1] (GTX113477) diluted at 1:1000.

Anti-IRF5 antibody [N1C1] used in Western Blot (WB). GTX113477

GTX113477 WB Image

Sample (30 ug of whole cell lysate)
A: HepG2
7.5% SDS PAGE
GTX113477 diluted at 1:1000

Anti-IRF5 antibody [N1C1] used in Western Blot (WB). GTX113477

GTX113477 WB Image

Mouse tissue extract (50 μg) was separated by 10% SDS-PAGE, and the membrane was blotted with IRF5 antibody [N1C1] (GTX113477) diluted at 1:500.

REFERENCE

Application Reference

Xu Q et al. J Biomed Nanotechnol 2021; 17 (3) : 466-476 Interferon Regulatory Factor 5 siRNA-Loaded Folate-Modified Cationic Liposomes for Acute Lung Injury Therapy.
Lim R et al. Reproduction 2018; (Epub) IRF5 is increased in laboring myometrium and regulates pro-labor mediators.
Application : WB, IHC-P
Reactivity : Human
Submit a Reference

REVIEW

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SDS
PBS.pdf
Glycerol.pdf
Thimerosal.pdf
Package List Price ($)
$ 399
$ 169