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IRF6 antibody, C-term

Anti-IRF6 antibody, C-term used in Western Blot (WB). GTX10925
Anti-IRF6 antibody, C-term used in IHC (Paraffin sections) (IHC-P). GTX10925
Anti-IRF6 antibody, C-term used in Western Blot (WB). GTX10925
Anti-IRF6 antibody, C-term used in IHC (Paraffin sections) (IHC-P). GTX10925

Cat. No. GTX10925

Host

 Goat

Clonality

 Polyclonal

Isotype

 IgG

Application

 WB, IHC-P

Reactivity

 Human
Package
100 μg ($399)
See all IRF6 products

APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Recommended Dilution
WB 0.2-0.6μg/ml
IHC-P 2-5μg/ml

Note :

IHC-P
Human Kidney shows strong nuclear staining in DCT and glomerulus.
Not tested in other applications.

Calculated MW

53 kDa. ( Note )

Positive Control

The peptide used to generate this antibody is available for purchase (GTX10925-PEP).

PROPERTIES

Form

Liquid

Buffer

TBS, 0.5% BSA

Preservative

0.02% Sodium azide

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

0.50 mg/ml (Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

Peptide with sequence C-TPSMQLPPALPPQ, from the C Terminus of the protein sequence according to NP_006138.1.

Purification

Purified by ammonium sulphate precipitation followed by antigen affinity chromatography

Conjugation

Unconjugated

Note

For laboratory research use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

Purchasers shall not, and agree not to enable third parties to, analyze, copy, reverse engineer or otherwise attempt to determine the structure or sequence of the product.

TARGET

Synonyms

interferon regulatory factor 6 , LPS , OFC6 , PIT , PPS , PPS1 , VWS , VWS1

Cellular Localization

Nucleus , Cytoplasm

Background

This gene encodes a member of the interferon regulatory transcription factor (IRF) family. Family members share a highly-conserved N-terminal helix-turn-helix DNA-binding domain and a less conserved C-terminal protein-binding domain. The encoded protein may be a transcriptional activator. Mutations in this gene can cause van der Woude syndrome and popliteal pterygium syndrome. Mutations in this gene are also associated with non-syndromic orofacial cleft type 6. Alternate splicing results in multiple transcript variants.[provided by RefSeq, May 2011]

Database

Research Area

DATA IMAGES

Anti-IRF6 antibody, C-term used in Western Blot (WB). GTX10925

GTX10925 WB Image

WB analysis of HEK293 overexpressing IRF6 (mock transfection in first lane) using GTX10925 IRF6 antibody, C-term.

Anti-IRF6 antibody, C-term used in IHC (Paraffin sections) (IHC-P). GTX10925

GTX10925 IHC-P Image

IHC-P analysis of human kidney using GTX10925 IRF6 antibody, C-term.
Antigen retrieval : citrate buffer pH 6
Dilution : 2.5μg/ml

Anti-IRF6 antibody, C-term used in Western Blot (WB). GTX10925

GTX10925 WB Image

WB analysis of human ovary lysate using GTX10925 IRF6 antibody, C-term.
Dilution : 0.2μg/ml
Loading : 35μg protein in RIPA buffer

Anti-IRF6 antibody, C-term used in IHC (Paraffin sections) (IHC-P). GTX10925

GTX10925 IHC-P Image

IHC-P analysis of human prostate using GTX10925 IRF6 antibody, C-term.
Antigen retrieval : citrate buffer pH 6
Dilution : 5μg/ml

REFERENCE

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REVIEW

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SDS
Sodium Azide.pdf
Package List Price ($)
$ 399