GeneTex
United States (US)

IRF6 antibody [C3], C-term

GTX104862_39708_IFA_mESC_19011506_765.jpg
GTX104862_39708_20160518_IHC-P_R_19011506_985.jpg
GTX104862_WB_19011506_718.jpg

Cat No. GTX104862

Host Rabbit
Clonality Polyclonal
Isotype IgG
Application WB, ICC/IF, IHC-P
Reactivity Human, Mouse, Rat
APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Dilution
WB 1:500-1:3000
ICC/IF 1:50-1:500
IHC-P 1:100-1:1000
Not tested in other applications.

Calculated MW

53 kDa. ( Note )

Positive Control

IRF6 tansfected 293T cell

Predict Reactivity

Rhesus Monkey(>80% identity)
PROPERTIES

Form

Liquid

Buffer

1XPBS, 10% Glycerol (pH7). 0.01% Thimerosal was added as a preservative.

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

1 mg/ml (Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

Carrier-protein conjugated synthetic peptide encompassing a sequence within the C-terminus region of human IRF6. The exact sequence is proprietary.

Purification

Purified by antigen-affinity chromatography.

Conjugation

Unconjugated

Note

For laboratory use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.
TARGET

Synonyms

Interferon Regulatory Factor 6,Lps,Ofc6,Pit,Pps,Pps1,Vws,Vws1,Irf6

Background

This gene encodes a member of the interferon regulatory transcription factor (IRF) family. Family members share a highly-conserved N-terminal helix-turn-helix DNA-binding domain and a less conserved C-terminal protein-binding domain. Mutations in this gene can cause van der Woude syndrome and popliteal pterygium syndrome. This protein is involved in palate formation. [provided by RefSeq]

Database

Research Area

DATA IMAGES
GTX104862_39708_IFA_mESC_19011506_765.jpg

GTX104862 ICC/IF Image

Immunofluorescence analysis of methanol-fixed mouse ESC, using IRF6(GTX104862) antibody at 1:100 dilution.

GTX104862_39708_20160518_IHC-P_R_19011506_985.jpg

GTX104862 IHC-P Image

IRF6 antibody [C3], C-term detects IRF6 protein at cytoplasm and nucleus in rat liver by immunohistochemical analysis.
Sample: Paraffin-embedded rat liver.
IRF6 antibody [C3], C-term (GTX104862) diluted at 1:500.

Antigen Retrieval: Citrate buffer, pH 6.0, 15 min

GTX104862_WB_19011506_718.jpg

GTX104862 WB Image

Western Blot analysis of IRF6 expression in transfected 293T cell line by IRF6 polyclonal antibody.
A: Non-transfected lysate.
B: IRF6 transfected lysate.
12% SDS PAGE
GTX104862 diluted at 1:500

REFERENCE
SDS
PBS.pdf
Glycerol.pdf
Thimerosal.pdf
Package List Price ($)
$ 319
$ 169