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IRF7 antibody [3D9]

Anti-IRF7 antibody [3D9] used in Western Blot (WB). GTX50051

Cat No. GTX50051

Host

Mouse

Clonality

Monoclonal

Clone Name

3D9

Isotype

IgG1

Application

WB, ELISA

Reactivity

Human
Package
100 μl ($319)

APPLICATION

Application Note

We recommend the following starting dilutions:
Western Blot: Use at 1:1,000~ 2,000.

Optimal working concentrations should be determined experimentally by the end user.

Calculated MW

54 kDa. ( Note )

PROPERTIES

Form

Liquid

Buffer

Supplied in Phosphate-Buffered Saline (pH 7.4) with 0.1% Sodium Azide.

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

1 mg/ml (Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

Recombinant human IRF-7 (1-150aa) purified from E. coli

Purification

By protein-G affinity chromatography

Conjugation

Unconjugated

Note

For laboratory use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

TARGET

Synonyms

Interferon Regulatory Factor 7 , Imd39 , Irf-7 , Irf-7H , Irf7A , Irf7B , Irf7C , Irf7H , Irf7

Cellular Localization

The phosphorylated and active form accumulates selectively in the nucleus.

Background

Interferon regulatory factor (IRF) 7 is a member of the interferon regulatory transcription factor family. IRF7 plays an essential role in virus-activated transcription of type I interferon alpha genes. Inducible expression of IRF7 is largely restricted to lymphoid tissue. Multiple IRF7 transcript variants have been identified, although the functional consequences of these have not yet been established.

Database

Research Area

DATA IMAGES

Anti-IRF7 antibody [3D9] used in Western Blot (WB). GTX50051

GTX50051 WB Image

Cell lysates of Jurkat (40ug) were resolved by SDS-PAGE, transferred to NC membrane and probed with anti-human IRF-7 (1:1,000). Proteins were visualized using a goat anti-mouse secondary antibody conjugated to HRP and an ECL detection system.

REFERENCE

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REVIEW

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SDS
Sodium Azide.pdf
Package List Price ($)
$ 319