APPLICATION
Application Note
*Optimal dilutions/concentrations should be determined by the researcher.
| Application |
Recommended Dilution |
| 1:500-1:3000 |
Not tested in other applications.
Calculated MW
Positive Control
THP-1 nuclear extract
Predict Reactivity
Mouse, Rat, Bovine, Pig, Rhesus Monkey(>80% identity)
PROPERTIES
Form
Liquid
Buffer
PBS, 10% Glycerol
Preservative
0.01% Thimerosal
Storage
Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.
Concentration
1 mg/ml (Please refer to the vial label for the specific concentration.)
Antigen Species
Human
Immunogen
Carrier-protein conjugated synthetic peptide encompassing a sequence within the C-terminus region of human IRF8. The exact sequence is proprietary.
Purification
Purified by antigen-affinity chromatography.
Conjugation
Unconjugated
RRID
AB_1240995
Note
For laboratory research use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.
Purchasers shall not, and agree not to enable third parties to, analyze, copy, reverse engineer or otherwise attempt to determine the structure or sequence of the product.
TARGET
Synonyms
interferon regulatory factor 8 , H-ICSBP , ICSBP , ICSBP1 , IMD32A , IMD32B , IRF-8
Cellular Localization
Nucleus
Background
Interferon consensus sequence-binding protein (ICSBP) is a transcription factor of the interferon (IFN) regulatory factor (IRF) family. Proteins of this family are composed of a conserved DNA-binding domain in the N-terminal region and a divergent C-terminal region that serves as the regulatory domain. The IRF family proteins bind to the IFN-stimulated response element (ISRE) and regulate expression of genes stimulated by type I IFNs, namely IFN-alpha and IFN-beta. IRF family proteins also control expression of IFN-alpha and IFN-beta-regulated genes that are induced by viral infection. [provided by RefSeq]
Database
Research Area
DATA IMAGES
![Anti-IRF8 antibody [C3], C-term used in Western Blot (WB). GTX106322](/templates/default/js/lightbox/images/loading.gif "Anti-IRF8 antibody [C3], C-term used in Western Blot (WB). GTX106322")
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GTX106322 WB Image
THP-1 whole cell and nuclear extracts (30 μg) were separated by 10% SDS-PAGE, and the membranes were blotted with IRF8 antibody [C3], C-term (GTX106322) diluted at 1:500 and competitor's antibody (sc-6058) diluted at 1:100. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.
*The competitor is not affiliated with GeneTex and does not endorse this product.
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REFERENCE
There are currently no references for IRF8 antibody [C3], C-term (GTX106322). Be the first to share your publications with this product.
REVIEW
There are currently no reviews for IRF8 antibody [C3], C-term (GTX106322). Be the first to share your experience with this product.
![Anti-IRF8 antibody [C3], C-term used in Western Blot (WB). GTX106322](/upload/website/prouct_img/small/GTX106322/GTX106322_39701_20171020_WB_Fraction_competitor_watermark_w_23060120_989.webp "Anti-IRF8 antibody [C3], C-term used in Western Blot (WB). GTX106322")
![Anti-IRF8 antibody [C3], C-term used in Western Blot (WB). GTX106322](/upload/website/prouct_img/normal/GTX106322/GTX106322_39701_20171020_WB_Fraction_competitor_watermark_w_23060120_989.webp "Anti-IRF8 antibody [C3], C-term used in Western Blot (WB). GTX106322")