GeneTex
United States (US)

IRF8 antibody [C3], C-term

Comparison Orthogonal
GTX106322_39701_20171020_WB_Fraction_competitor_watermark_19011506_543.jpg

Cat No. GTX106322

Host Rabbit
Clonality Polyclonal
Isotype IgG
Application WB
Reactivity Human
APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Dilution
WB 1:500-1:3000
Not tested in other applications.

Calculated MW

48 kDa. ( Note )

Positive Control

THP-1 nuclear extract

Predict Reactivity

Mouse, Rat, Bovine, Pig, Rhesus Monkey(>80% identity)
PROPERTIES

Form

Liquid

Buffer

1XPBS, 10% Glycerol (pH7). 0.01% Thimerosal was added as a preservative.

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

1mg/ml(Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

Carrier-protein conjugated synthetic peptide encompassing a sequence within the C-terminus region of human IRF8. The exact sequence is proprietary.

Purification

Purified by antigen-affinity chromatography.

Conjugation

Unconjugated

Note

For laboratory use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.
TARGET

Synonyms

H-ICSBP antibody, ICSBP antibody, ICSBP1 antibody, IRF-8 antibody, IRF8 antibody, interferon consensus sequence binding protein 1 antibody, interferon regulatory factor 8 antibody

Cellular Localization

Nucleus

Background

Interferon consensus sequence-binding protein (ICSBP) is a transcription factor of the interferon (IFN) regulatory factor (IRF) family. Proteins of this family are composed of a conserved DNA-binding domain in the N-terminal region and a divergent C-terminal region that serves as the regulatory domain. The IRF family proteins bind to the IFN-stimulated response element (ISRE) and regulate expression of genes stimulated by type I IFNs, namely IFN-alpha and IFN-beta. IRF family proteins also control expression of IFN-alpha and IFN-beta-regulated genes that are induced by viral infection. [provided by RefSeq]

Database

Research Area

DATA IMAGES
GTX106322_39701_20171020_WB_Fraction_competitor_watermark_19011506_543.jpg

GTX106322 WB Image

Comparison Orthogonal

THP-1 whole cell and nuclear extracts (30 µg) were separated by 10% SDS-PAGE, and the membranes were blotted with IRF8 antibody [C3], C-term (GTX106322) diluted at 1:500 and competitor's antibody (sc-6058) diluted at 1:100. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.
*The competitor is not affiliated with GeneTex and does not endorse this product.

SDS
PBS.pdf
Glycerol.pdf
Thimerosal.pdf
Package List Price ($)
$ 319
$ 169