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IRF8 antibody

Anti-IRF8 antibody used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX31696
Anti-IRF8 antibody used in Western Blot (WB). GTX31696

Cat. No. GTX31696

Host

 Rabbit

Clonality

 Polyclonal

Isotype

 IgG

Application

 WB, ICC/IF, ELISA

Reactivity

 Human, Mouse, Rat
Package
100 μg ($399)
See all IRF8 products

APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Recommended Dilution
WB 1 μg/mL
ICC/IF 10 μg/mL
ELISA Assay dependent
Not tested in other applications.

Calculated MW

48 kDa. ( Note )

Positive Control

K-562 Cell Slide(GTX25535) , Human Thymus Tissue Lysate

PROPERTIES

Form

Liquid

Buffer

PBS

Preservative

0.02% Sodium azide

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

1 mg/ml (Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

IRF8 antibody was raised against a peptide corresponding to 16 amino acids near the carboxy terminus of human IRF8.The immunogen is located within the last 50 amino acids of IRF8.

Purification

Purified by antigen-affinity chromatography

Conjugation

Unconjugated

Note

For laboratory research use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

Purchasers shall not, and agree not to enable third parties to, analyze, copy, reverse engineer or otherwise attempt to determine the structure or sequence of the product.

TARGET

Synonyms

interferon regulatory factor 8 , H-ICSBP , ICSBP , ICSBP1 , IMD32A , IMD32B , IRF-8

Cellular Localization

Nucleus,Cytoplasm

Background

Interferon consensus sequence-binding protein (ICSBP) is a transcription factor of the interferon (IFN) regulatory factor (IRF) family. Proteins of this family are composed of a conserved DNA-binding domain in the N-terminal region and a divergent C-terminal region that serves as the regulatory domain. The IRF family proteins bind to the IFN-stimulated response element (ISRE) and regulate expression of genes stimulated by type I IFNs, namely IFN-alpha and IFN-beta. IRF family proteins also control expression of IFN-alpha and IFN-beta-regulated genes that are induced by viral infection. [provided by RefSeq, Jul 2008]

Database

Research Area

DATA IMAGES

Anti-IRF8 antibody used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX31696

GTX31696 ICC/IF Image

ICC/IF analysis of K562 cells using GTX31696 IRF8 antibody.
Working concentration : 10 μg/ml

Anti-IRF8 antibody used in Western Blot (WB). GTX31696

GTX31696 WB Image

WB analysis of human thymus tissue lysate in (A) the presence and (B) absence of blocking peptide using GTX31696 IRF8 antibody.
Working concentration : 1μg/ml

REFERENCE

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REVIEW

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SDS
PBS.pdf
Sodium Azide.pdf
Package List Price ($)
$ 399