APPLICATION
Application Note
*Optimal dilutions/concentrations should be determined by the researcher.
| Application |
Recommended Dilution |
| 1 - 2 μg/mL |
| 2.5 μg/mL |
| Assay dependent |
Not tested in other applications.
Calculated MW
Positive Control
A-20 Cell Slide(GTX25541) , Mouse Brain Tissue Lysate(GTX27934)
Product Note
Two isoforms of IRGC are known to exist; this antibody will recognize both isoforms.
PROPERTIES
Form
Liquid
Buffer
PBS
Preservative
0.02% Sodium azide
Storage
Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.
Concentration
1 mg/ml (Please refer to the vial label for the specific concentration.)
Antigen Species
Human
Immunogen
IRGC antibody was raised against a 12 amino acid synthetic peptide near the carboxy terminus of human IRGC.The immunogen is located within the last 50 amino acids of IRGC.
Purification
Purified by antigen-affinity chromatography
Conjugation
Unconjugated
Note
For laboratory research use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.
Purchasers shall not, and agree not to enable third parties to, analyze, copy, reverse engineer or otherwise attempt to determine the structure or sequence of the product.
TARGET
Synonyms
immunity related GTPase cinema , CINEMA , IFGGE , IRGC1 , Iigp5 , R30953_1
Background
Immunity-related GTPases (IRG) (also known as p47 GTPases) are a family of GTPase proteins found in vertebrates, which play critical roles in mediating innate resistance to intracellular pathogens. IRG genes have been found in a number of mammals and lower species including mice, rats, zebrafish and humans. Most of the mouse genes contain interferon-stimulated response elements which mediate transcriptional activation by IFNs. In humans, only two IRG genes have been found: human IRGC encodes a full-length IRG protein that, like the mouse homologue, is constitutively expressed in testis, while human IRGM encodes a considerably truncated protein that is constitutively expressed in cultured cells including some macrophage cell lines. As the two human genes IRGC and IRGM are not subject to IFN control, it has been suggested that the host resistance mechanism supported by IRG proteins in the mouse is lacking in humans. Two isoforms of IRGC are known to exist; this antibody will recognize both isoforms.
Database
DATA IMAGES
|
GTX85258 ICC/IF Image
ICC/IF analysis of A-20 cells using GTX85258 IRGC antibody.
Working concentration : 20 μg/ml
|
|
|
GTX85258 ICC/IF Image
ICC/IF analysis of A-20 cells using GTX85258 IRGC antibody.
Working concentration : 2.5 μg/ml
|
|
|
GTX85258 WB Image
WB analysis of mouse brain tissue lysate using GTX85258 IRGC antibody.
Working concentration : (A) 1 and (B) 2 μg/ml
|
REFERENCE
There are currently no references for IRGC antibody (GTX85258). Be the first to share your publications with this product.
REVIEW
There are currently no reviews for IRGC antibody (GTX85258). Be the first to share your experience with this product.
