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IRGM antibody

Anti-IRGM antibody used in IHC (Paraffin sections) (IHC-P). GTX85039
Anti-IRGM antibody used in IHC (Paraffin sections) (IHC-P). GTX85039
Anti-IRGM antibody used in Western Blot (WB). GTX85039

Cat. No. GTX85039

Host

 Rabbit

Clonality

 Polyclonal

Isotype

 IgG

Application

 WB, IHC-P, ELISA

Reactivity

 Human, Mouse, Rat
Package
100 μg ($399)
See all IRGM products

APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Recommended Dilution
WB 1 - 2 μg/mL
IHC-P 5 μg/mL
ELISA Assay dependent
Not tested in other applications.

Calculated MW

20 kDa. ( Note )

Positive Control

SK-N-SH Cell Lysate(GTX23956)

PROPERTIES

Form

Liquid

Buffer

PBS

Preservative

0.02% Sodium azide

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

1 mg/ml (Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

IRGM antibody was raised against a 17 amino acid synthetic peptide near the amino terminus of the human IRGM.The immunogen is located within amino acids 30 - 80 of IRGM.

Purification

Purified by antigen-affinity chromatography

Conjugation

Unconjugated

Note

For laboratory research use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

Purchasers shall not, and agree not to enable third parties to, analyze, copy, reverse engineer or otherwise attempt to determine the structure or sequence of the product.

TARGET

Synonyms

immunity related GTPase M , IFI1 , IRGM1 , LRG-47 , LRG47

Cellular Localization

Golgi apparatus membrane,Cell membrane

Background

Autophagy, the process of bulk degradation of cellular proteins through an autophagosomic-lysosomal pathway is important for normal growth control and may be defective in tumor cells. It is involved in the preservation of cellular nutrients under starvation conditions as well as the normal turnover of cytosolic components. Two of the strongest hits implicate genes IRGM and ATG16L1, which encode proteins thought to be critical to the autophagy pathway and being significantly associated with Crohn’s disease. In mouse, IRGM belongs to a family of gamma-interferon-induced GTP-binding proteins of approximately 48 kDa. Murine IRGM induces autophagy and generates large autolysosomal organelles as a mechanism for the elimination of intracellular Mycobacterium tuberculosis. Human IRGM is also involved in autophagy and plays a role in the control of intracellular pathogens and in the reduction of intracellular bacillary load.

Database

Research Area

DATA IMAGES

Anti-IRGM antibody used in IHC (Paraffin sections) (IHC-P). GTX85039

GTX85039 IHC-P Image

IHC-P analysis of human brain tissue using GTX85039 IRGM antibody.
Working concentration : 20 μg/ml

Anti-IRGM antibody used in IHC (Paraffin sections) (IHC-P). GTX85039

GTX85039 IHC-P Image

IHC-P analysis of human thymus tissue using GTX85039 IRGM antibody.
Working concentration : 5 μg/ml

Anti-IRGM antibody used in Western Blot (WB). GTX85039

GTX85039 WB Image

WB analysis of SK-N-SH cell lysate using GTX85039 IRGM antibody.
Working concentration : (A) 1 and (B) 2 μg/ml

REFERENCE

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REVIEW

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SDS
PBS.pdf
Sodium Azide.pdf
Package List Price ($)
$ 399