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IRS1 antibody [C2C3], C-term

Anti-IRS1 antibody [C2C3], C-term used in Western Blot (WB). GTX113213
Anti-IRS1 antibody [C2C3], C-term used in Western Blot (WB). GTX113213
Anti-IRS1 antibody [C2C3], C-term used in IHC (Paraffin sections) (IHC-P). GTX113213

Cat. No. GTX113213

Reference 1

Host

 Rabbit

Clonality

 Polyclonal

Isotype

 IgG

Application

 WB, IHC-P

Reactivity

 Human
Package
100 μl ($399),
25 μl ($169)
See all IRS1 products

APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Recommended Dilution
WB 1:500-1:20000
IHC-P 1:100-1:1000
Not tested in other applications.

Calculated MW

132 kDa. ( Note )

Positive Control

A431 , MDA-MB-231

Predict Reactivity

Pig(>80% identity)

PROPERTIES

Form

Liquid

Buffer

PBS, 1% BSA, 20% Glycerol

Preservative

0.01% Thimerosal

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

1 mg/ml (Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

Recombinant protein encompassing a sequence within the C-terminus region of human IRS1. The exact sequence is proprietary.

Purification

Purified by antigen-affinity chromatography.

Conjugation

Unconjugated

RRID

AB_10730236

Note

For laboratory research use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

Purchasers shall not, and agree not to enable third parties to, analyze, copy, reverse engineer or otherwise attempt to determine the structure or sequence of the product.

TARGET

Synonyms

insulin receptor substrate 1 , HIRS-1

Background

This gene encodes a protein which is phosphorylated by insulin receptor tyrosine kinase. Mutations in this gene are associated with type II diabetes and susceptibility to insulin resistance. [provided by RefSeq]

Database

Research Area

DATA IMAGES

Anti-IRS1 antibody [C2C3], C-term used in Western Blot (WB). GTX113213

GTX113213 WB Image

Sample (30 ug of whole cell lysate)
A: A431
5% SDS PAGE
GTX113213 diluted at 1:10000

Anti-IRS1 antibody [C2C3], C-term used in Western Blot (WB). GTX113213

GTX113213 WB Image

Various whole cell extracts (30 μg) were separated by 5% SDS-PAGE, and the membrane was blotted with IRS1 antibody [C2C3], C-term (GTX113213) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

Anti-IRS1 antibody [C2C3], C-term used in IHC (Paraffin sections) (IHC-P). GTX113213

GTX113213 IHC-P Image

IRS1 antibody [C2C3], C-term detects IRS1 protein at cytoplasm on human lung carcinoma by immunohistochemical analysis.
Sample: Paraffin-embedded lung carcinoma.
IRS1 antibody [C2C3], C-term (GTX113213) dilution: 1:500.

Antigen Retrieval: Trilogy™ (EDTA based, pH 8.0) buffer, 15min

REFERENCE

Application Reference

Chang TC et al. Pharmacol Res 2021; 163 : 105298 Ugonin J improves metabolic disorder and ameliorates nonalcoholic fatty liver disease by regulating the AMPK/AKT signaling pathway.
Application : WB
Reactivity : Human
Submit a Reference

REVIEW

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SDS
PBS.pdf
Glycerol.pdf
Thimerosal.pdf
Package List Price ($)
$ 399
$ 169