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IRS1 antibody

Anti-IRS1 antibody used in IHC (Paraffin sections) (IHC-P). GTX50550
Anti-IRS1 antibody used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX50550
Anti-IRS1 antibody used in Western Blot (WB). GTX50550

Cat. No. GTX50550

Host

 Rabbit

Clonality

 Polyclonal

Isotype

 IgG

Application

 WB, ICC/IF, IHC-P

Reactivity

 Human
Package
100 μl ($399)
See all IRS1 products

APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Recommended Dilution
WB 1:500-1:1000
ICC/IF 1:100-1:200
IHC-P 1:50-1:100
Not tested in other applications.

Calculated MW

132 kDa. ( Note )

PROPERTIES

Form

Liquid

Buffer

PBS (without Mg²⁺ and Ca²⁺) pH7.4, 150mM NaCl, 50% Glycerol

Preservative

0.02% Sodium azide

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

1 mg/ml (Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

Peptide sequence around aa.634~638 (P-M-S-P-K) derived from human IRS1.

Purification

Purified by antigen-affinity chromatography.

Conjugation

Unconjugated

Note

For laboratory research use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

Purchasers shall not, and agree not to enable third parties to, analyze, copy, reverse engineer or otherwise attempt to determine the structure or sequence of the product.

TARGET

Synonyms

insulin receptor substrate 1 , HIRS-1

Background

This gene encodes a protein which is phosphorylated by insulin receptor tyrosine kinase. Mutations in this gene are associated with type II diabetes and susceptibility to insulin resistance. [provided by RefSeq, Nov 2009]

Database

Research Area

DATA IMAGES

Anti-IRS1 antibody used in IHC (Paraffin sections) (IHC-P). GTX50550

GTX50550 IHC-P Image

IHC-P analysis of human breast carcinoma tissue using GTX50550 IRS1 antibody.
Left : Primary antibody
Right : Primary antibody pre-incubated with the antigen specific peptide

Anti-IRS1 antibody used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX50550

GTX50550 ICC/IF Image

ICC/IF analysis of methanol-fixed HeLa cells using GTX50550 IRS1 antibody.

Anti-IRS1 antibody used in Western Blot (WB). GTX50550

GTX50550 WB Image

WB analysis of extracts from 293 cells using GTX50550 IRS1 antibody.

REFERENCE

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REVIEW

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SDS
PBS.pdf
Glycerol.pdf
Sodium Azide.pdf
Package List Price ($)
$ 399