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IRS1 (phospho Ser312) antibody

Orthogonal
Anti-IRS1 (phospho Ser312) antibody used in Western Blot (WB). GTX24865
Anti-IRS1 (phospho Ser312) antibody used in Western Blot (WB). GTX24865
Anti-IRS1 (phospho Ser312) antibody used in Western Blot (WB). GTX24865
Anti-IRS1 (phospho Ser312) antibody used in Western Blot (WB). GTX24865

Cat. No. GTX24865

Reference 1

Host

 Rabbit

Clonality

 Polyclonal

Isotype

 IgG

Application

 WB, IHC-P

Reactivity

 Human, Mouse
Package
50 μl ($399)
See all IRS1 products

APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Recommended Dilution
WB Assay dependent
IHC-P Assay dependent
Not tested in other applications.

Calculated MW

132 kDa. ( Note )

PROPERTIES

Form

Liquid

Buffer

PBS, 0.1% BSA, 50% Glycerol

Preservative

0.05% Sodium azide

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

Batch dependent (Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

The antiserum was produced against a chemically synthesized phosphopeptide derived from a region of human IRS-1 that contains serine 312. The sequence is conserved in mouse, rat and pig.

Purification

Purified by antigen-affinity chromatography

Conjugation

Unconjugated

RRID

AB_380451

Note

For laboratory research use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

Purchasers shall not, and agree not to enable third parties to, analyze, copy, reverse engineer or otherwise attempt to determine the structure or sequence of the product.

TARGET

Synonyms

insulin receptor substrate 1 , HIRS-1

Background

This gene encodes a protein which is phosphorylated by insulin receptor tyrosine kinase. Mutations in this gene are associated with type II diabetes and susceptibility to insulin resistance. [provided by RefSeq, Nov 2009]

Database

Research Area

DATA IMAGES

Anti-IRS1 (phospho Ser312) antibody used in Western Blot (WB). GTX24865

GTX24865 WB Image

Orthogonal

WB (peptide competition) analysis of CHO-T cells transiently transfected with wild-type human IRS-1 and treated with 100 ng/mL TPA for 30 minutes using GTX24865 IRS1 (phospho Ser312) antibody prior incubated with no peptide (Lane 1~3) , the non-phosphopeptide corresponding to the phosphopeptide immunogen (Lane 4), a generic phosphoserine-containing peptide (Lane 5), or the phosphopeptide immunogen (Lane 6) . The data show that only the immunogen phosphopeptide blocks the signal, demonstrating the specificity of the antibody. The membrane treated with phosphatase (Lane 1) eliminates the signal further verifying that the antibody is phospho-specific.

Anti-IRS1 (phospho Ser312) antibody used in Western Blot (WB). GTX24865

GTX24865 WB Image

Orthogonal

The data was published in the journal Sci Rep in 2017.PMID: 29038536

Anti-IRS1 (phospho Ser312) antibody used in Western Blot (WB). GTX24865

GTX24865 WB Image

Orthogonal

The data was published in the journal Sci Rep in 2017.PMID: 29038536

Anti-IRS1 (phospho Ser312) antibody used in Western Blot (WB). GTX24865

GTX24865 WB Image

Orthogonal

The data was published in the journal Sci Rep in 2017.PMID: 29038536

REFERENCE

Application Reference

Chen X et al. Sci Rep 2017; 7 (1) : 13248 hydrogen sulphide treatment increases * sensitivity and improves oxidant metabolism through the camkkbeta-ampk pathway in pa-induced ir c2c12 cells.
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REVIEW

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SDS
PBS.pdf
Glycerol.pdf
Sodium Azide.pdf
Package List Price ($)
$ 399