IHC-P analysis of human breast carcinoma tissue using GTX24776 IRS1 (phospho Ser616) antibody.
Right : Primary antibody
Left : Negative control without primary antibody
Antigen retrieval : 10mM sodium citrate (pH 6.0), microwaved for 8-15 min
Dilution : 1:100

WB analysis of 293T cells transfected with wild-type human IRS-1 and treated with 100ng/mL TPA for 30 minutes using GTX24776 IRS1 (phospho Ser616) antibody prior incubated with the non-phosphopeptide corresponding to the phosphopeptide immunogen (Lane 2), a generic phosphoserine-containing peptide (Lane 3), or the phosphopeptide immunogen (Lane 4).

IHC-P analysis of mouse skeletal muscle tissue using GTX24776 IRS1 (phospho Ser616) antibody.
Right : Primary antibody
Left : Negative control without primary antibody
Antigen retrieval : 10mM sodium citrate (pH 6.0), microwaved for 8-15 min
Dilution : 1:20