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IRS1 (phospho Ser616) antibody

Orthogonal
Anti-IRS1 (phospho Ser616) antibody used in IHC (Paraffin sections) (IHC-P). GTX24776
Anti-IRS1 (phospho Ser616) antibody used in Western Blot (WB). GTX24776
Anti-IRS1 (phospho Ser616) antibody used in IHC (Paraffin sections) (IHC-P). GTX24776

Cat. No. GTX24776

Host

 Rabbit

Clonality

 Polyclonal

Isotype

 IgG

Application

 WB, ICC/IF, IHC-P, FACS, IHC

Reactivity

 Human, Mouse, Rat
Package
50 μl ($399)
See all IRS1 products

APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Recommended Dilution
WB 1:1000
ICC/IF Assay dependent
IHC-P 1:10-1:100
FACS 1:20
IHC Assay dependent
Not tested in other applications.

Calculated MW

132 kDa. ( Note )

PROPERTIES

Form

Liquid

Buffer

PBS, 0.1% BSA, 50% Glycerol

Preservative

0.05% Sodium azide

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

Batch dependent (Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

The antiserum was produced against a chemically synthesized phosphopeptide derived from a region of IRS-1 that contains serine 616. The sequence is conserved in many species including human, mouse and rat.

Purification

Purified by antigen-affinity chromatography

Conjugation

Unconjugated

Note

For laboratory research use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

Purchasers shall not, and agree not to enable third parties to, analyze, copy, reverse engineer or otherwise attempt to determine the structure or sequence of the product.

TARGET

Synonyms

insulin receptor substrate 1 , HIRS-1

Background

This gene encodes a protein which is phosphorylated by insulin receptor tyrosine kinase. Mutations in this gene are associated with type II diabetes and susceptibility to insulin resistance. [provided by RefSeq, Nov 2009]

Database

Research Area

DATA IMAGES

Anti-IRS1 (phospho Ser616) antibody used in IHC (Paraffin sections) (IHC-P). GTX24776

GTX24776 IHC-P Image

IHC-P analysis of mouse skeletal muscle tissue using GTX24776 IRS1 (phospho Ser616) antibody.
Right : Primary antibody
Left : Negative control without primary antibody
Antigen retrieval : 10mM sodium citrate (pH 6.0), microwaved for 8-15 min
Dilution : 1:20

Anti-IRS1 (phospho Ser616) antibody used in Western Blot (WB). GTX24776

GTX24776 WB Image

Orthogonal

WB analysis of 293T cells transfected with wild-type human IRS-1 and treated with 100ng/mL TPA for 30 minutes using GTX24776 IRS1 (phospho Ser616) antibody prior incubated with the non-phosphopeptide corresponding to the phosphopeptide immunogen (Lane 2), a generic phosphoserine-containing peptide (Lane 3), or the phosphopeptide immunogen (Lane 4).

Anti-IRS1 (phospho Ser616) antibody used in IHC (Paraffin sections) (IHC-P). GTX24776

GTX24776 IHC-P Image

IHC-P analysis of human breast carcinoma tissue using GTX24776 IRS1 (phospho Ser616) antibody.
Right : Primary antibody
Left : Negative control without primary antibody
Antigen retrieval : 10mM sodium citrate (pH 6.0), microwaved for 8-15 min
Dilution : 1:100

REFERENCE

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REVIEW

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SDS
PBS.pdf
Glycerol.pdf
Sodium Azide.pdf
Package List Price ($)
$ 399