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IRS1 (phospho Ser636) antibody

Anti-IRS1 (phospho Ser636) antibody used in IHC (Paraffin sections) (IHC-P). GTX32400
Anti-IRS1 (phospho Ser636) antibody used in Western Blot (WB). GTX32400
Anti-IRS1 (phospho Ser636) antibody used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX32400

Cat No. GTX32400

Host Rabbit
Clonality Polyclonal
Isotype IgG
Application WB, ICC/IF, IHC-P
Reactivity Human, Mouse
Package
100 μl ($319)
APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Dilution
WB 1:500 - 1:1000
ICC/IF 1:100 - 1:500
IHC-P 1:100 - 1:200
Not tested in other applications.

Calculated MW

132 kDa. ( Note )
PROPERTIES

Form

Liquid

Buffer

0.42% Potassium phosphate, 0.87% Sodium chloride pH 7.3, 30% glycerol, 0.01% sodium azide

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20º C or below. Avoid multiple freeze-thaw cycles.

Antigen Species

Human

Immunogen

KLH-conjugated synthetic peptide encompassing a sequence within the center region of IRS1. The exact sequence is proprietary.

Purification

Purified by antigen-affinity chromatography

Conjugation

Unconjugated

Note

For laboratory use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.
TARGET

Synonyms

insulin receptor substrate 1 , HIRS-1

Background

This gene encodes a protein which is phosphorylated by insulin receptor tyrosine kinase. Mutations in this gene are associated with type II diabetes and susceptibility to insulin resistance. [provided by RefSeq, Nov 2009]

Database

Research Area

DATA IMAGES
Anti-IRS1 (phospho Ser636) antibody used in IHC (Paraffin sections) (IHC-P). GTX32400

GTX32400 IHC-P Image

IHC-P analysis of formalin fixed human lung cancer tissue section using GTX32400 IRS1 (phospho Ser636) antibody.
Antigen retrieval : Heat mediated antigen retrieval with sodium citrate buffer (pH 6.0)

Anti-IRS1 (phospho Ser636) antibody used in Western Blot (WB). GTX32400

GTX32400 WB Image

WB analysis of UV-treated HeLa (A), SH-SY5Y (B), NIH3T3 (C) whole cell lysates using GTX32400 IRS1 (phospho Ser636) antibody.

Anti-IRS1 (phospho Ser636) antibody used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX32400

GTX32400 ICC/IF Image

ICC/IF analysis of formalin-fixed MCF7 cells using GTX32400 IRS1 (phospho Ser636) antibody.
Red : Primary antibody
Blue : DAPI
Permeabilization : 0.1% Triton X-100 in TBS for 5-10 minutes

REFERENCE
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REVIEW
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