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IRS1 (phospho Ser636) antibody

Anti-IRS1 (phospho Ser636) antibody used in Western Blot (WB). GTX32400
Anti-IRS1 (phospho Ser636) antibody used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX32400
Anti-IRS1 (phospho Ser636) antibody used in IHC (Paraffin sections) (IHC-P). GTX32400

Cat. No. GTX32400

Reference 2

Host

 Rabbit

Clonality

 Polyclonal

Isotype

 IgG

Application

 WB, ICC/IF, IHC-P

Reactivity

 Human, Mouse
Package
100 μl ($399)
See all IRS1 products

APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Recommended Dilution
WB 1:500 - 1:1000
ICC/IF 1:100 - 1:500
IHC-P 1:100 - 1:200
Not tested in other applications.

Calculated MW

132 kDa. ( Note )

PROPERTIES

Form

Liquid

Buffer

0.42% Potassium Phosphate, 0.87% NaCl, 30% Glycerol

Preservative

0.01% Sodium azide

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

Batch dependent (Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

KLH-conjugated synthetic peptide encompassing a sequence within the center region of IRS1. The exact sequence is proprietary.

Purification

Purified by antigen-affinity chromatography

Conjugation

Unconjugated

Note

For laboratory research use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

Purchasers shall not, and agree not to enable third parties to, analyze, copy, reverse engineer or otherwise attempt to determine the structure or sequence of the product.

TARGET

Synonyms

insulin receptor substrate 1 , HIRS-1

Background

This gene encodes a protein which is phosphorylated by insulin receptor tyrosine kinase. Mutations in this gene are associated with type II diabetes and susceptibility to insulin resistance. [provided by RefSeq, Nov 2009]

Database

Research Area

DATA IMAGES

Anti-IRS1 (phospho Ser636) antibody used in Western Blot (WB). GTX32400

GTX32400 WB Image

WB analysis of UV-treated HeLa (A), SH-SY5Y (B), NIH3T3 (C) whole cell lysates using GTX32400 IRS1 (phospho Ser636) antibody.

Anti-IRS1 (phospho Ser636) antibody used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX32400

GTX32400 ICC/IF Image

ICC/IF analysis of formalin-fixed MCF7 cells using GTX32400 IRS1 (phospho Ser636) antibody.
Red : Primary antibody
Blue : DAPI
Permeabilization : 0.1% Triton X-100 in TBS for 5-10 minutes

Anti-IRS1 (phospho Ser636) antibody used in IHC (Paraffin sections) (IHC-P). GTX32400

GTX32400 IHC-P Image

IHC-P analysis of formalin fixed human lung cancer tissue section using GTX32400 IRS1 (phospho Ser636) antibody.
Antigen retrieval : Heat mediated antigen retrieval with sodium citrate buffer (pH 6.0)

REFERENCE

Application Reference

Ilaria Zuliani et al. Int J Mol Sci 2021; 22 (7) High-Fat Diet Leads to Reduced Protein O-GlcNAcylation and Mitochondrial Defects Promoting the Development of Alzheimer's Disease Signatures.
Application : WB
Reactivity : Mouse
Zuliani I et al. Neurotherapeutics 2020; (Epub) The Dysregulation of OGT/OGA Cycle Mediates Tau and APP Neuropathology in Down Syndrome.
Application : WB
Reactivity : Mouse
Submit a Reference

REVIEW

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SDS
Glycerol.pdf
Sodium Azide.pdf
Package List Price ($)
$ 399