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IRS1 (phospho Ser636) antibody

Anti-IRS1 (phospho Ser636) antibody used in Western Blot (WB). GTX50265
Anti-IRS1 (phospho Ser636) antibody used in IHC (Paraffin sections) (IHC-P). GTX50265

Cat No. GTX50265

Host

Rabbit

Clonality

Polyclonal

Isotype

IgG

Application

WB, IHC-P

Reactivity

Human, Mouse
Package
100 μl ($319)

APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Dilution
WB 1:500-1:1000
IHC-P 1:50-1:100
Not tested in other applications.

Calculated MW

132 kDa. ( Note )

Predict Reactivity

Rat(>80% identity)

PROPERTIES

Form

Liquid

Buffer

PBS (without Mg²⁺ and Ca²⁺) pH 7.4, 150mM NaCl, 0.02% sodium azide, 50% glycerol

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

1 mg/ml (Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

Peptide sequence around phosphorylation site of serine 636 (P-M-S(p)-P-K) derived from human IRS1.

Purification

Purified by antigen-affinity chromatography. Non-phospho specific antibodies were removed by chromatogramphy using non-phosphopeptide.

Conjugation

Unconjugated

Note

For laboratory use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

TARGET

Synonyms

insulin receptor substrate 1 , HIRS-1

Background

This gene encodes a protein which is phosphorylated by insulin receptor tyrosine kinase. Mutations in this gene are associated with type II diabetes and susceptibility to insulin resistance. [provided by RefSeq, Nov 2009]

Database

Research Area

DATA IMAGES

Anti-IRS1 (phospho Ser636) antibody used in Western Blot (WB). GTX50265

GTX50265 WB Image

WB analysis of extracts from 3T3 cells untreated or treated with Insulin using GTX50265 IRS1 (phospho Ser636) antibody.

Anti-IRS1 (phospho Ser636) antibody used in IHC (Paraffin sections) (IHC-P). GTX50265

GTX50265 IHC-P Image

IHC-P analysis of human breast carcinoma tissue using GTX50265 IRS1 (phospho Ser636) antibody.
Left : Primary antibody
Right : Primary antibody pre-incubated with the antigen specific peptide

REFERENCE

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REVIEW

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SDS
PBS.pdf
Glycerol.pdf
Sodium Azide.pdf