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IRS2 antibody, C-term

Anti-IRS2 antibody, C-term used in Western Blot (WB). GTX81291
Anti-IRS2 antibody, C-term used in IHC (Paraffin sections) (IHC-P). GTX81291
Anti-IRS2 antibody, C-term used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX81291
Anti-IRS2 antibody, C-term used in Western Blot (WB). GTX81291

Cat. No. GTX81291

Host

 Rabbit

Clonality

 Polyclonal

Isotype

 IgG

Application

 WB, ICC/IF, IHC-P

Reactivity

 Human
Package
400 μl ($399)
See all IRS2 products

APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Recommended Dilution
WB 1:1000
ICC/IF 1:10-1:50
IHC-P 1:50-1:100
Not tested in other applications.

Calculated MW

137 kDa. ( Note )

PROPERTIES

Form

Liquid

Buffer

PBS

Preservative

0.09% Sodium azide

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

Batch dependent (Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

KLH conjugated synthetic peptide between 1309-1338 amino acids from the C-terminal region of human IRS2.

Purification

Saturated Ammonium Sulfate (SAS) precipitation followed by dialysis against PBS.

Conjugation

Unconjugated

Note

For laboratory research use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

Purchasers shall not, and agree not to enable third parties to, analyze, copy, reverse engineer or otherwise attempt to determine the structure or sequence of the product.

TARGET

Synonyms

insulin receptor substrate 2 , IRS-2

Cellular Localization

Cytoplasm, cytosol

Background

This gene encodes the insulin receptor substrate 2, a cytoplasmic signaling molecule that mediates effects of insulin, insulin-like growth factor 1, and other cytokines by acting as a molecular adaptor between diverse receptor tyrosine kinases and downstream effectors. The product of this gene is phosphorylated by the insulin receptor tyrosine kinase upon receptor stimulation, as well as by an interleukin 4 receptor-associated kinase in response to IL4 treatment. [provided by RefSeq, Jul 2008]

Database

Research Area

DATA IMAGES

Anti-IRS2 antibody, C-term used in Western Blot (WB). GTX81291

GTX81291 WB Image

WB analysis of HeLa whole cell lysate using GTX81291 IRS2 antibody, C-term.
Loading : 20 μg per lane
Dilution : 1:1000

Anti-IRS2 antibody, C-term used in IHC (Paraffin sections) (IHC-P). GTX81291

GTX81291 IHC-P Image

IHC-P analysis of human hepatocarcinoma using GTX81291 IRS2 antibody, C-term.

Anti-IRS2 antibody, C-term used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX81291

GTX81291 ICC/IF Image

ICC/IF analysis of HeLa cells using GTX81291 IRS2 antibody, C-term.
Green : IRS2
Red : Actin filament
Fixation : 4% paraformaldehyde (PFA) (20 min)
Permeabilization : Triton X-100 (0.1%, 10 min)
Dilution : 1:25

Anti-IRS2 antibody, C-term used in Western Blot (WB). GTX81291

GTX81291 WB Image

WB analysis of 293 cell lysate (35ug/lane) using GTX81291 IRS2 antibody, C-term.

REFERENCE

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REVIEW

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SDS
PBS.pdf
Sodium Azide.pdf
Package List Price ($)
$ 399