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IRS4 antibody

Anti-IRS4 antibody used in IHC (Paraffin sections) (IHC-P). GTX130865
Anti-IRS4 antibody used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX130865
Anti-IRS4 antibody used in Western Blot (WB). GTX130865

Cat. No. GTX130865

Host

 Rabbit

Clonality

 Polyclonal

Isotype

 IgG

Application

 WB, ICC/IF, IHC-P

Reactivity

 Human
Package
100 μl ($399),
25 μl ($169)
See all IRS4 products

APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Recommended Dilution
WB 1:500-1:3000
ICC/IF 1:100-1:1000
IHC-P Assay dependent
Not tested in other applications.

Calculated MW

134 kDa. ( Note )

Positive Control

293T

PROPERTIES

Form

Liquid

Buffer

PBS, 1% BSA, 20% Glycerol

Preservative

0.025% ProClin 300

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

0.4 mg/ml (Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

Recombinant protein encompassing a sequence within the C-terminus region of human IRS4. The exact sequence is proprietary.

Purification

Purified by antigen-affinity chromatography.

Conjugation

Unconjugated

RRID

AB_2886370

Note

For laboratory research use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

Purchasers shall not, and agree not to enable third parties to, analyze, copy, reverse engineer or otherwise attempt to determine the structure or sequence of the product.

TARGET

Synonyms

insulin receptor substrate 4 , IRS-4 , PY160

Cellular Localization

Cell membrane

Background

IRS4 encodes the insulin receptor substrate 4, a cytoplasmic protein that contains many potential tyrosine and serine/threonine phosphorylation sites. Tyrosine-phosphorylated IRS4 protein has been shown to associate with cytoplasmic signalling molecules that contain SH2 domains. The IRS4 protein is phosphorylated by the insulin receptor tyrosine kinase upon receptor stimulation.. [provided by RefSeq, Jul 2008]

Database

Research Area

DATA IMAGES

Anti-IRS4 antibody used in IHC (Paraffin sections) (IHC-P). GTX130865

GTX130865 IHC-P Image

IRS4 antibody detects IRS4 protein at cell membrane and cytoplasm by immunohistochemical analysis.
Sample: Paraffin-embedded human hepatocellular carcinoma.
IRS4 stained by IRS4 antibody (GTX130865) diluted at 1:500.
Antigen Retrieval: Citrate buffer, pH 6.0, 15 min

Anti-IRS4 antibody used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX130865

GTX130865 ICC/IF Image

IRS4 antibody detects IRS4 protein at cell membrane by immunofluorescent analysis.
Sample: 293T cells were fixed in 4% paraformaldehyde at RT for 15 min.
Green: IRS4 stained by IRS4 antibody (GTX130865) diluted at 1:500.
Blue: Hoechst 33342 staining.

Anti-IRS4 antibody used in Western Blot (WB). GTX130865

GTX130865 WB Image

Various whole cell extracts (30 μg) were separated by 5% SDS-PAGE, and the membrane was blotted with IRS4 antibody (GTX130865) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

REFERENCE

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REVIEW

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SDS
PBS.pdf
Glycerol.pdf
Proclin.pdf
Package List Price ($)
$ 399
$ 169