ISCU antibody [N1C3]

Cat. No. GTX115709
1
Cat. No.  GTX115709
Overexpression
Anti-ISCU antibody [N1C3] used in Western Blot (WB). GTX115709
GTX115709 WB Image

The observed M.W. is based on the publication: PMID: 26560363

Various whole cell extracts (30 μg) were separated by 15% SDS-PAGE, and the membrane was blotted with ISCU antibody [N1C3] (GTX115709) diluted at 1:500. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

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Anti-ISCU antibody [N1C3] used in Western Blot (WB). GTX115709
GTX115709 WB Image
Overexpression

Non-transfected (–) and transfected (+) 293T whole cell extracts (30 μg) were separated by 15% SDS-PAGE, and the membrane was blotted with ISCU antibody [N1C3] (GTX115709) diluted at 1:5000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

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Anti-ISCU antibody [N1C3] used in IHC (Paraffin sections) (IHC-P). GTX115709
GTX115709 IHC-P Image

ISCU antibody [N1C3] detects ISCU protein at cytosol on mouse muscle by immunohistochemical analysis.
Sample: Paraffin-embedded mouse muscle.
ISCU antibody [N1C3] (GTX115709) dilution: 1:500.

Antigen Retrieval: Trilogy™ (EDTA based, pH 8.0) buffer, 15min

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Anti-ISCU antibody [N1C3] used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX115709
GTX115709 ICC/IF Image

ISCU antibody [N1C3] detects ISCU protein at mitochondria by immunofluorescent analysis.
Sample: HeLa cells were fixed in ice-cold MeOH for 5 min.
Green: ISCU stained by ISCU antibody [N1C3] (GTX115709) diluted at 1:500.
Blue: Hoechst 33342 staining.

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Anti-ISCU antibody [N1C3] used in IHC (Paraffin sections) (IHC-P). GTX115709
GTX115709 IHC-P Image

Immunohistochemical analysis of paraffin-embedded A549 xenograft, using ISCU(GTX115709) antibody at 1:500 dilution.
Antigen Retrieval: Trilogy™ (EDTA based, pH 8.0) buffer, 15min

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Anti-ISCU antibody [N1C3] used in Western Blot (WB). GTX115709
Anti-ISCU antibody [N1C3] used in Western Blot (WB). GTX115709
Anti-ISCU antibody [N1C3] used in IHC (Paraffin sections) (IHC-P). GTX115709
Anti-ISCU antibody [N1C3] used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX115709
Anti-ISCU antibody [N1C3] used in IHC (Paraffin sections) (IHC-P). GTX115709
This ISCU antibody [N1C3] (GTX115709) is a rabbit polyclonal antibody validated for detection of ISCU by WB, ICC/IF, and IHC-P. Reactivity has been shown in Human and Mouse.
  • Host
    Rabbit
  • Clonality
    Polyclonal
  • Isotype
    IgG
  • Applications
    WB ICC/IF IHC-P
  • Reactivity
    Human, Mouse
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