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ISG15 antibody [3E5]

Anti-ISG15 antibody [3E5] used in Western Blot (WB). GTX50062

Cat. No. GTX50062

Host

 Mouse

Clonality

 Monoclonal

Clone Name

 3E5

Isotype

 IgG1

Application

 WB, ELISA

Reactivity

 Human
Package
100 μl ($399)
See all ISG15 products

APPLICATION

Application Note

We recommend the following starting dilutions:
Western Blot: Use at 1:1,000 ~ 2,000.

Optimal working concentrations should be determined experimentally by the end user.

Calculated MW

18 kDa. ( Note )

PROPERTIES

Form

Liquid

Buffer

PBS

Preservative

0.1% Sodium azide

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

1 mg/ml (Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

Recombinant human ISG15 (1-157aa) purified from E. coli

Purification

By protein-G affinity chromatography

Conjugation

Unconjugated

Note

For laboratory research use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

Purchasers shall not, and agree not to enable third parties to, analyze, copy, reverse engineer or otherwise attempt to determine the structure or sequence of the product.

TARGET

Synonyms

ISG15 ubiquitin like modifier , G1P2 , IFI15 , IMD38 , IP17 , UCRP , hUCRP

Cellular Localization

Cytoplasm , Secreted

Background

The protein encoded by this gene is a ubiquitin-like protein that is conjugated to intracellular target proteins upon activation by interferon-alpha and interferon-beta. Several functions have been ascribed to the encoded protein, including chemotactic activity towards neutrophils, direction of ligated target proteins to intermediate filaments, cell-to-cell signaling, and antiviral activity during viral infections. While conjugates of this protein have been found to be noncovalently attached to intermediate filaments, this protein is sometimes secreted. [provided by RefSeq, Dec 2012]

Database

Research Area

DATA IMAGES

Anti-ISG15 antibody [3E5] used in Western Blot (WB). GTX50062

GTX50062 WB Image

Cell lysates of HeLa(each 50ug) were resolved by SDS-PAGE, transferred to PVDF membrane and probed with anti-human ISG15 (1:1,000). Proteins were visualized using a goat anti-mouse secondary antibody conjugated to HRP and an ECL detection system

REFERENCE

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SDS
Sodium Azide.pdf
Package List Price ($)
$ 399