Wild-type (WT) and ISG15 knockout (KO) HeLa cell extracts (30 μg) were separated by 15% SDS-PAGE, and the membrane was blotted with ISG15 antibody [HL2017] (GTX637918) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

ISG15 antibody [HL2017] detects ISG15 protein at cytoplasm by immunohistochemical analysis.
Sample: Paraffin-embedded cat brain.
ISG15 stained by ISG15 antibody [HL2017] (GTX637918) diluted at 1:100.
Antigen Retrieval: Citrate buffer, pH 6.0, 15 min

Whole cell extract (30 μg) was separated by 15% SDS-PAGE, and the membrane was blotted with ISG15 antibody [HL2017] (GTX637918) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody, and the signal was developed with Trident ECL plus-Enhanced.

Untreated (–) and treated (+) A549 whole cell extract (30 μg) were separated by 15% SDS-PAGE, and the membrane was blotted with ISG15 antibody [HL2017] (GTX637918) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

ISG15 antibody [HL2017] detects ISG15 protein at cytoplasm and nucleus by immunofluorescent analysis.
Sample: HepG2 cells were fixed in ice-cold MeOH for 5 min.
Green: ISG15 stained by ISG15 antibody [HL2017] (GTX637918) diluted at 1:500.
Blue: Fluoroshield with DAPI (GTX30920).
Scale bar= 10μm.

Various tissue extracts (50 μg) were separated by 15% SDS-PAGE, and the membrane was blotted with ISG15 antibody [HL2017] (GTX637918) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.