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ISG15 antibody

Anti-ISG15 antibody used in IHC (Paraffin sections) (IHC-P). GTX32686
Anti-ISG15 antibody used in Western Blot (WB). GTX32686
Anti-ISG15 antibody used in IHC (Paraffin sections) (IHC-P). GTX32686

Cat. No. GTX32686

Reference 1

Host

 Rabbit

Clonality

 Polyclonal

Isotype

 IgG

Application

 WB, IHC-P

Reactivity

 Human, Rat
Package
100 μl ($399)
See all ISG15 products

APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Recommended Dilution
WB 1:500 - 1:2000
IHC-P 1:50 - 1:200
Not tested in other applications.

Calculated MW

18 kDa. ( Note )

PROPERTIES

Form

Liquid

Buffer

PBS, 50% Glycerol

Preservative

0.02% Sodium azide

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

Batch dependent (Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

Recombinant fusion protein containing a sequence corresponding to amino acids 1-165 of human ISG15 (NP_005092.1).

Purification

Purified by affinity chromatography

Conjugation

Unconjugated

RRID

AB_2887675

Note

For laboratory research use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

Purchasers shall not, and agree not to enable third parties to, analyze, copy, reverse engineer or otherwise attempt to determine the structure or sequence of the product.

TARGET

Synonyms

ISG15 ubiquitin like modifier , G1P2 , IFI15 , IMD38 , IP17 , UCRP , hUCRP

Cellular Localization

Cytoplasm,Secreted

Background

The protein encoded by this gene is a ubiquitin-like protein that is conjugated to intracellular target proteins upon activation by interferon-alpha and interferon-beta. Several functions have been ascribed to the encoded protein, including chemotactic activity towards neutrophils, direction of ligated target proteins to intermediate filaments, cell-to-cell signaling, and antiviral activity during viral infections. While conjugates of this protein have been found to be noncovalently attached to intermediate filaments, this protein is sometimes secreted. [provided by RefSeq, Dec 2012]

Database

Research Area

DATA IMAGES

Anti-ISG15 antibody used in IHC (Paraffin sections) (IHC-P). GTX32686

GTX32686 IHC-P Image

IHC-P analysis of rat ovary tissue using GTX32686 ISG15 antibody.
Dilution : 1:100

Anti-ISG15 antibody used in Western Blot (WB). GTX32686

GTX32686 WB Image

WB analysis of various sample lysates using GTX32686 ISG15 antibody.
Dilution : 1:1000
Loading : 25μg per lane

Anti-ISG15 antibody used in IHC (Paraffin sections) (IHC-P). GTX32686

GTX32686 IHC-P Image

IHC-P analysis of human thyroid cancer tissue using GTX32686 ISG15 antibody.
Dilution : 1:100

REFERENCE

Application Reference

Lo Cigno I et al. J Virol 2020; 94 (4) Human Papillomavirus E7 Oncoprotein Subverts Host Innate Immunity via SUV39H1-Mediated Epigenetic Silencing of Immune Sensor Genes.
Application : WB
Reactivity : Human
Submit a Reference

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SDS
PBS.pdf
Glycerol.pdf
Sodium Azide.pdf
Package List Price ($)
$ 399