Various whole cell extracts (30 μg) were separated by 12% SDS-PAGE, and the membrane was blotted with ISG20 antibody (GTX114499) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody, and the signal was developed with Trident ECL plus-Enhanced.

Mouse tissue extract (50 μg) was separated by 12% SDS-PAGE, and the membrane was blotted with ISG20 antibody (GTX114499) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody, and the signal was developed with Trident ECL plus-Enhanced.

Immunohistochemical analysis of paraffin-embedded BT483 xenograft, using ISG20(GTX114499) antibody at 1:500 dilution.
Antigen Retrieval: Citrate buffer, pH 6.0, 15 min

Immunofluorescence analysis of paraformaldehyde-fixed HeLa, using ISG20(GTX114499) antibody at 1:500 dilution.