GeneTex

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United States (US)

IST1 antibody

Cat. No. GTX101972

Reference 5

Host

 Rabbit

Clonality

 Polyclonal

Isotype

 IgG

Application

 WB

Reactivity

 Mouse
Package
100 μl ($399),
25 μl ($169)
See all IST1 products

APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Recommended Dilution
WB Assay dependent
Not tested in other applications.

Calculated MW

40 kDa. ( Note )

Predict Reactivity

Human(>80% identity)

PROPERTIES

Form

Liquid

Buffer

PBS, 40% Glycerol

Preservative

0.01% Thimerosal

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

1 mg/ml (Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

Recombinant protein encompassing a sequence within the center region of human IST1. The exact sequence is proprietary.

Purification

Purified by antigen-affinity chromatography.

Conjugation

Unconjugated

RRID

AB_1241005

Note

For laboratory research use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

Purchasers shall not, and agree not to enable third parties to, analyze, copy, reverse engineer or otherwise attempt to determine the structure or sequence of the product.

TARGET

Synonyms

IST1 factor associated with ESCRT-III , CHMP8 , OLC1

Cellular Localization

Cytoplasmic vesicle

Background

Proposed to be involved in specific functions of the ESCRT machinery. Is required for efficient abscission during cytokinesis, but not for HIV-1 budding. The involvment in the MVB pathway is not established. Involved in recruiting VPS4A and/or VPS4B to the midbody of dividing cells.

Database

Research Area

REFERENCE

Application Reference

Varland S et al. Nat Commun 2023; 14 (1) : 6774 N-terminal acetylation shields proteins from degradation and promotes age-dependent motility and longevity.
Feng Q et al. Autophagy 2020; 16 (4) : 641-658 MAPT/Tau accumulation represses autophagy flux by disrupting IST1-regulated ESCRT-III complex formation: a vicious cycle in Alzheimer neurodegeneration.
Chang CL et al. J Cell Biol 2019; 218 (8) : 2583-2599 Spastin tethers lipid droplets to peroxisomes and directs fatty acid trafficking through ESCRT-III.
Chi-Lun Chang et al. bioRxiv 2019; (Epub) Spastin tethers lipid droplets to peroxisomes and directs fatty acid trafficking through ESCRT-III.
Renvoise B et al. Hum Mol Genet 2012; 21 (16) : 3604-18 Spg20-/- mice reveal multimodal functions for Troyer syndrome protein spartin in lipid droplet maintenance, cytokinesis and BMP signaling.
Application : WB
Reactivity : Mouse
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SDS
PBS.pdf
Glycerol.pdf
Thimerosal.pdf
Package List Price ($)
$ 399
$ 169