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ITCH antibody [GT1248]

Recombinant antibody KO/KD
Anti-ITCH antibody [GT1248] used in Western Blot (WB). GTX02845
Anti-ITCH antibody [GT1248] used in Western Blot (WB). GTX02845
Anti-ITCH antibody [GT1248] used in Western Blot (WB). GTX02845
Anti-ITCH antibody [GT1248] used in Western Blot (WB). GTX02845
Anti-ITCH antibody [GT1248] used in Western Blot (WB). GTX02845

Cat. No. GTX02845

Reference 1

Host

 Rabbit

Clonality

 Monoclonal

Clone Name

 GT1248

Isotype

 IgG

Application

 WB

Reactivity

 Human, Mouse, Rat
Package
100 μl ($419)
See all ITCH products

APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Recommended Dilution
WB 1:500 - 1:2000
Not tested in other applications.

Calculated MW

103 kDa. ( Note )

PROPERTIES

Form

Liquid

Buffer

PBS, 0.05% BSA, 50% Glycerol

Preservative

0.02% Sodium azide

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, we recommend adding 50% glycerol, then aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

1.6 mg/ml (Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

A synthesized peptide derived from human ITCH

Purification

Purified by antigen-affinity chromatography

Conjugation

Unconjugated

Note

For laboratory research use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

Purchasers shall not, and agree not to enable third parties to, analyze, copy, reverse engineer or otherwise attempt to determine the structure or sequence of the product.

TARGET

Synonyms

itchy E3 ubiquitin protein ligase , ADMFD , AIF4 , AIP4 , NAPP1

Cellular Localization

Cell membrane , Cytoplasm , Nucleus , Endosome membrane

Background

This gene encodes a member of the Nedd4 family of HECT domain E3 ubiquitin ligases. HECT domain E3 ubiquitin ligases transfer ubiquitin from E2 ubiquitin-conjugating enzymes to protein substrates, thus targeting specific proteins for lysosomal degradation. The encoded protein plays a role in multiple cellular processes including erythroid and lymphoid cell differentiation and the regulation of immune responses. Mutations in this gene are a cause of syndromic multisystem autoimmune disease. Alternatively spliced transcript variants encoding multiple isoforms have been observed for this gene. [provided by RefSeq, Mar 2012]

Database

Research Area

DATA IMAGES

Anti-ITCH antibody [GT1248] used in Western Blot (WB). GTX02845

GTX02845 WB Image

Whole cell extract (30 μg) was separated by 7.5% SDS-PAGE, and the membrane was blotted with ITCH antibody [GT1248] (GTX02845) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

Anti-ITCH antibody [GT1248] used in Western Blot (WB). GTX02845

GTX02845 WB Image

Various whole cell extracts (30 μg) were separated by 7.5% SDS-PAGE, and the membrane was blotted with ITCH antibody [GT1248] (GTX02845) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

Anti-ITCH antibody [GT1248] used in Western Blot (WB). GTX02845

GTX02845 WB Image

WB analysis of various samples using GTX02845 ITCH antibody [GT1248].
Dilution : 1:1000
Loading : 25μg

Anti-ITCH antibody [GT1248] used in Western Blot (WB). GTX02845

GTX02845 WB Image

WB analysis of various samples using GTX02845 ITCH antibody [GT1248].
Dilution : 1:1000
Loading : 25μg

Anti-ITCH antibody [GT1248] used in Western Blot (WB). GTX02845

GTX02845 WB Image

KO/KD

WB analysis of wide type (WT) and ITCH knockout (KO) 293T cells using GTX02845 ITCH antibody [GT1248].
Dilution : 1:1000
Loading : 25μg

REFERENCE

Application Reference

Hsiao-Chin Chou et al. J Biomed Sci 2022; 29 (1) : 40 DUSP3 regulates phosphorylation-mediated degradation of occludin and is required for maintaining epithelial tight junction.
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REVIEW

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Package List Price ($)
$ 419