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ITCH (phospho Tyr420) antibody

Anti-ITCH (phospho Tyr420) antibody used in IHC (Paraffin sections) (IHC-P). GTX55343

Cat. No. GTX55343

Host

 Rabbit

Clonality

 Polyclonal

Isotype

 IgG

Application

 IHC-P

Reactivity

 Human
Package
100 μl ($399)
See all ITCH products

APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Recommended Dilution
IHC-P 1:50-1:100
Not tested in other applications.

Calculated MW

103 kDa. ( Note )

Predict Reactivity

Mouse(>80% identity)

PROPERTIES

Form

Liquid

Buffer

PBS (without Mg²⁺ and Ca²⁺) pH7.4, 150mM NaCl, 50% Glycerol

Preservative

0.02% Sodium azide

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

1 mg/ml (Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

Peptide sequence around phosphorylation site of tyrosine 420(F-I-Y(p)-G-N) derived from human ITCH.

Purification

Purified by antigen-affinity chromatography. Non-phospho specific antibodies were removed by chromatogramphy using non-phosphopeptide.

Conjugation

Unconjugated

Note

For laboratory research use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

Purchasers shall not, and agree not to enable third parties to, analyze, copy, reverse engineer or otherwise attempt to determine the structure or sequence of the product.

TARGET

Synonyms

itchy E3 ubiquitin protein ligase , ADMFD , AIF4 , AIP4 , NAPP1

Cellular Localization

Cell membrane,Cytoplasm,Nucleus,Endosome membrane

Background

This gene encodes a member of the Nedd4 family of HECT domain E3 ubiquitin ligases. HECT domain E3 ubiquitin ligases transfer ubiquitin from E2 ubiquitin-conjugating enzymes to protein substrates, thus targeting specific proteins for lysosomal degradation. The encoded protein plays a role in multiple cellular processes including erythroid and lymphoid cell differentiation and the regulation of immune responses. Mutations in this gene are a cause of syndromic multisystem autoimmune disease. Alternatively spliced transcript variants encoding multiple isoforms have been observed for this gene. [provided by RefSeq, Mar 2012]

Database

Research Area

DATA IMAGES

Anti-ITCH (phospho Tyr420) antibody used in IHC (Paraffin sections) (IHC-P). GTX55343

GTX55343 IHC-P Image

IHC-P analysis of human brain tissue using GTX55343 ITCH (phospho Tyr420) antibody.
Left : Primary antibody
Right : Primary antibody pre-incubated with the antigen specific peptide

REFERENCE

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REVIEW

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SDS
PBS.pdf
Glycerol.pdf
Sodium Azide.pdf
Package List Price ($)
$ 399